Immunocytochemical localization of proANF 1-30, proANF 31-67 and atrial natriuretic factor in the kidney.
Immunocytochemical localization of proANF 1-30, proANF 31-67 and atrial natriuretic factor in the kidney.
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DOI：
10.1038/ki.1992.46
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发表时间：
1992-02
影响因子：
19.6
通讯作者：
G. Ramirez;S. Saba;J. Dietz;D. Vesely
中科院分区：
文献类型：
--
作者：
G. Ramirez;S. Saba;J. Dietz;D. Vesely
心钠素原1-30、31-67和心钠素原在肾脏的免疫细胞化学定位ProANF [1-30前30个氨基酸（a.a）126 a.a心房利钠因子（ANF）激素原]，ProANF 31-67（a.a31 -67）以及心房利钠因子（a.a.99 -126），的ANF激素原定位于亚-用免疫过氧化物酶染色观察水合和脱水大鼠肾脏近端小管的卷部和直部刷状缘。免疫荧光研究显示，这些肽中的每一种，尤其是ProANF 31-67，对近端和远端小管中的核周区域具有强烈的偏好。ProANF 1-30、31-67和99-126（即ANF）也通过免疫过氧化物酶和免疫荧光染色定位于皮质集合管、肾小球、管周和间质血管。ProANF 31-67免疫过氧化物酶染色在小和大间质动脉的弹性纤维中特别显著。通过放射免疫分析，大鼠肾脏中ANF激素原的N-末端和C-末端的免疫识别表明存在整个激素原。肾脏中N-末端的浓度为1.0 ± 0.03 ng/g肾重，而ANF激素原浓度的C-末端为0.4 ± 0.01 ng/g肾组织。N末端和C末端ANF激素原肽的刷状缘下和核周位置的这些发现表明，心房利尿钠因子激素原可能在近端小管中合成和/或相应的肽被近端小管捕获，但也在较小程度上被远端小管捕获。
Immunocytochemical localization of proANF 1-30, proANF 31-67 and atrial natriuretic factor in the kidney. ProANF [1-30 first 30 amino acids (a.a) of the 126 a.a atrial natriuretic factor (ANF) prohormone], ProANF 31-67 (a.a 31–67) as well as atrial natriuretic factor (a.a 99-126), of the ANF prohormone localized to the sub-brush border of the pars convoluta and pars rectus of the proximal tubules of hydrated and dehydrated rat kidneys with immunoperoxidase staining. Immunofluorescent studies revealed that each of these peptides and especially ProANF 31-67 had a strong predilection for the perinuclear region in the proximal and distal tubules. ProANFs 1-30, 31-67, and 99-126 (that is, ANF) also localized with both immunoperoxidase and immunofluorescent staining to the cortical collecting ducts, glomeruli, peritubular, and interstitial blood vessels. ProANF 31-67 immunoperoxidase staining was particularly striking in the elastica of the small and large interstitial arteries. The whole prohormone being present was suggested by immunological recognition in the rat kidney of both the N-terminus and C-terminus of the ANF prohormone by radioimmunoassays. The concentration of the N-terminus in the kidney was 1.0 ± 0.03 ng/g of kidney weight, while the C-terminus of the ANF prohormone concentration was 0.4 ± 0.01 ng/g of kidney tissue. These findings of a sub-brush border and perinuclear location of the N-terminal and C-terminal ANF prohormone peptides suggest that the atrial natriuretic factor prohormone may be synthesized in and/or the respective peptides are captured by the proximal tubules, but also to a lesser extent by the distal tubules of the kidney.