Ultrastructural analysis of the central terminals of primary sensory neurones labelled by transganglionic transport of Bandeiraea simplicifolia I-isolectin B4
Ultrastructural analysis of the central terminals of primary sensory neurones labelled by transganglionic transport of Bandeiraea simplicifolia I-isolectin B4
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DOI：
10.1016/j.neuroscience.2004.05.008
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发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Plenderleith, MB
中科院分区：
文献类型：
--
作者：
Gerke, MB;Plenderleith, MB
在这项研究中，初级感觉神经元的超微结构的外观标记的植物凝集素Bandeiraea simplicifolia I-异凝集素B-4（BSI-B-4）注射到周围神经已被检查在大鼠。逆行标记的神经元的胞体的电子显微镜显示凝集素与细胞质囊泡的内表面相关联，支持的前提下，BSI-B-4到感觉神经元的摄取是通过受体介导的内吞作用的过程。脊髓的光镜和电镜分析显示transganglionically运输凝集素在无髓轴突的背外侧索和轴突终端集中主要在第二层的背角。对1377个轴突终末的详细分析表明，大多数轴突终末呈球状，周围有多达14个未标记的轮廓。这些研究结果表明，初级感觉神经元transganglionically运输BSI-B-4有一个突触的超微结构类似，这是以前报道的无髓鞘初级感觉神经元。此外，似乎这些神经元的轴突末梢受到广泛的调制。凝集素标记的轴突终末的囊泡内容物的检查发现，大多数含有小的无颗粒囊泡，而大颗粒囊泡，只是偶尔观察到。这些发现支持了表达BSI-B-4结合位点的神经元群体与含有神经活性肽的神经元群体相当不同的建议。总之，目前的研究结果表明，凝集素BSI-B-4可以用作小直径初级感觉神经元的亚群的组织学标记物，并提供进一步的证据，这种凝集素作为一个有用的工具，在疼痛的研究潜力。(C)2004年IBRO。由爱思唯尔有限公司出版。保留所有权利。
In this study the ultrastructural appearance of primary sensory neurones labelled by the injection of the plant lectin Bandeiraea simplicifolia I-isolectin B-4 (BSI-B-4) into a peripheral nerve has been examined in the rat. Electron microscopy of the somata of retrogradely labelled neurones showed the lectin to be associated with the inner surface of cytoplasmic vesicles, supporting the premise that the uptake of BSI-B-4 into sensory neurones is by the process of receptor-mediated endocytosis. Light and electron microscopic analysis of the spinal cord revealed transganglionically transported lectin in unmyelinated axons in the dorsolateral funiculus and axon terminals concentrated mainly within lamina II of the dorsal horn. Detailed analysis of 1377 of these axon terminals revealed that the majority were glomerular in shape and surrounded by up to 14 other unlabelled profiles. These findings suggest that primary sensory neurones which transganglionically transport BSI-B-4 have a synaptic ultrastructure similar to that which has been previously reported for unmyelinated primary sensory neurones. Moreover, it appears that the axon terminals of these neurones are subjected to extensive modulation. Examination of the vesicle content of lectin labelled axon terminals revealed that the majority contained small agranular vesicles while large granular vesicles were observed only occasionally. These findings support the suggestion that the populations of neurones expressing binding sites for BSI-B-4 are fairly distinct from those containing neuroactive peptides. In conclusion, the results of the current study suggest that the lectin BSI-B-4 can be used as a histological marker for a subpopulation of small diameter primary sensory neurones and provide further evidence for the potential of this lectin as a useful tool in the study of pain. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.