CAROTID BARORECEPTOR MODULATION OF DIAPHRAGM AND ABDOMINAL MUSCLE-ACTIVITY IN CAT
CAROTID BARORECEPTOR MODULATION OF DIAPHRAGM AND ABDOMINAL MUSCLE-ACTIVITY IN CAT
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DOI：
10.1152/jappl.1974.36.1.12
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发表时间：
1974-01-01
影响因子：
3.3
通讯作者：
BISHOP, B
中科院分区：
文献类型：
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作者：
BISHOP, B
方法成年猫腹腔内注射Dial和Ciba公司生产的Dial（0.6ml/kg体重）。气管插管，自主呼吸。通过连接至斯坦森压力传感器（P23 AC）的股动脉插管测量全身动脉压。用细双极丝电极插入胸骨附着处的隔膜和暴露的斜腹肌中检测M USC e I动作电位。来自两块肌肉的动作电位通过两个Grass EMG前置放大器（型号5 P3）放大和“整合”，并记录在Grass测谎仪（型号7）的单独通道上。在示波器上视觉监测两块肌肉的EMG，并通过音频放大器和扬声器进行听觉监测。为了诱发主动呼气，通过将气管插管连接到一个大容器（55加仑的鼓），对动物进行连续正压呼吸，其中压力可以在-20至+20 cmHg 0之间调节。在水压计上监测储层压力。通过频繁冲洗滚筒和仅在短时间暴露于压力下期间让动物呼吸到滚筒和从滚筒呼吸来避免再呼吸效应。颈动脉窦压力感受器放电减少闭塞颈总动脉收紧夹具或结扎事先放置在血管周围。很快就发现，右颈总动脉的闭塞不能引起可重复的反射反应，除非动脉最初被清洁和剥离。图1改编自Boss和绿色的论文（10，1 I，2 I，ZZ），显示猫右颈动脉中的压力感受器不限于颈动脉分叉，而是具有更宽的分布。通过组织学和电生理学技术，Boss和绿色证明了在上级甲状腺动脉水平的压力感受器，接受迷走神经分支的神经支配，以及在从锁骨下动脉到颈内动脉和颈外动脉分叉的右总动脉沿着的其他几个区域。每个压力感受器
METHODSTen adult cats were anesthetized with an intraperitoneal injection of Dial with urethanl(Ciba; 0.6 ml/kg body wt). The trachea was cannulated and breathing was spontaneous. Systemic arterial pressure was measured from a femoral arterial cannula connected to a Statham pressure transducer (P23AC). M USC e I action potentials were detected with fine bipolar wire electrodes inserted into the diaphragm at its sternal attachment and into the exposed oblique abdominal muscle. The action potentials from the two muscles were amplified and “integrated” by two Grass EMG preamplifiers (model 5P3) and recorded on separate channels of a Grass polygraph(model 7). The EMGs of the two muscles were monitored visually on an oscilloscope and aurally via an audioamplifier and speaker, To evoke active expiration the animal was subjected to continuous positive-pressure breathing by connecting the tracheal cannula to a large reservoir, a 55-gal drum, in which the pressure could be adjusted from-20 to+ 20 cmHg0. Reservoir pressure was monitored on a water manometer. Rebreathing effects were avoided by frequent flushing of the drum and by having the animal breathe to and from the drum only during the short exposures to the ventilatory stress. Carotid sinus baroreceptor firing was decreased by occluding the common carotid arteries by tightening clamps or ligatures previously placed around the vessels. It soon became apparent that occlusion of the right common carotid artery failed to evoke reproducible reflex responses unless the artery was initially cleaned and stripped. Figure 1, adapted from Boss and Green’s papers (10, 1 I, 2 I, ZZ), shows that baroreceptors in the right carotid of the cat are not confined to the carotid bifurcation, but have a much wider distribution. By histological and electrophysiological techniques Boss and Green demonstrated baroreceptors at the level of the superior thyroid artery, receiving innervation from a branch of the vagus nerve, and in several other areas along the right common artery from the subclavian artery to the external and internal carotid bifurcation. Each baroreceptor