Target-dependent use of coreleased inhibitory transmitters at central synapses
Target-dependent use of coreleased inhibitory transmitters at central synapses
复制标题
DOI：
10.1523/jneurosci.1500-05.2005
复制
发表时间：
2005-07-13
影响因子：
5.3
通讯作者：
Dieudonné, S
中科院分区：
文献类型：
--
作者：
Dugué, GP;Dumoulin, A;Dieudonné, S
混合神经元共同释放GABA和甘氨酸是脊椎动物后脑抑制性传递的一种普遍模式。然而，很少有人知道的混合抑制网络的功能组织。高尔基体细胞，小脑颗粒层的主要抑制性中间神经元，已被证明含有GABA和甘氨酸。我们在这里表明，在前庭小脑，高尔基体细胞接触颗粒细胞和单极刷细胞，这是兴奋性中继中间神经元前庭传入。颗粒细胞中的IPSC仅由GABA（A）受体介导，而高尔基体细胞对单极刷状细胞的抑制由甘氨酸能电流主导。我们进一步证明，一个单一的高尔基体细胞可以执行纯GABA能抑制颗粒细胞和纯甘氨酸能抑制单极刷状细胞。这种特化是由靶细胞GABAA和甘氨酸受体的差异表达而不是由GAB（A）和甘氨酸在突触前末梢中的分离引起的。因此，在中枢神经系统中使用突触后选择的共同释放的快速递质，以增加个体神经元输出的多样性，并在混合抑制网络中实现靶特异性信号传导。
Corelease of GABA and glycine by mixed neurons is a prevalent mode of inhibitory transmission in the vertebrate hindbrain. However, little is known of the functional organization of mixed inhibitory networks. Golgi cells, the main inhibitory interneurons of the cerebellar granular layer, have been shown to contain GABA and glycine. We show here that, in the vestibulocerebellum, Golgi cells contact both granule cells and unipolar brush cells, which are excitatory relay interneurons for vestibular afferences. Whereas IPSCs in granule cells are mediated by GABA(A) receptors only, Golgi cell inhibition of unipolar brush cells is dominated by glycinergic currents. We further demonstrate that a single Golgi cell can perform pure GABAergic inhibition of granule cells and pure glycinergic inhibition of unipolar brush cells. This specialization results from the differential expression of GABAA and glycine receptors by target cells and not from a segregation of GAB(A) and glycine in presynaptic terminals. Thus, postsynaptic selection of coreleased fast transmitters is used in the CNS to increase the diversity of individual neuronal outputs and achieve target-specific signaling in mixed inhibitory networks.