Human immunodeficiency virus type 1 vectors efficiently transduce human hematopoietic stem cells.
Human immunodeficiency virus type 1 vectors efficiently transduce human hematopoietic stem cells.
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人类免疫缺陷病毒1型载体可有效转导人类造血干细胞。
DOI：
10.1128/jvi.72.7.5781-5788.1998
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发表时间：
1998
影响因子：
5.4
通讯作者：
Brown,PO
中科院分区：
文献类型：
--
作者：
Sutton,RE;Wu,HT;Rigg,R;Böhnlein,E;Brown,PO
与逆转录病毒相比，慢病毒作为基因转移媒介具有潜在的优势，因为它们可以感染非分裂细胞。我们在这里证明了基于人类免疫缺陷病毒1型（HIV-1）的载体在转导纯化的人类造血干细胞方面是非常有效的。通过标记基因表达或整合前病毒的PCR测量，转导率超过50%，并且转导似乎与有丝分裂无关。构建HIV-1的衍生物以优化载体，并且需要删除大部分Vif和Vpr，以确保转导细胞的长期持久性，并相对稳定地表达标记基因产物。这些结果扩展了慢病毒载体系统的实用性。
Lentiviruses are potentially advantageous compared to oncoretroviruses as gene transfer agents because they can infect nondividing cells. We demonstrate here that human immunodeficiency virus type 1 (HIV-1)-based vectors were highly efficient in transducing purified human hematopoietic stem cells. Transduction rates, measured by marker gene expression or by PCR of the integrated provirus, exceeded 50%, and transduction appeared to be independent of mitosis. Derivatives of HIV-1 were constructed to optimize the vector, and a deletion of most of Vif and Vpr was required to ensure the long-term persistence of transduced cells with relatively stable expression of the marker gene product. These results extend the utility of this lentivirus vector system.