Skeletal muscle IL-6 and regulation of liver metabolism during high-fat diet and exercise training
Skeletal muscle IL-6 and regulation of liver metabolism during high-fat diet and exercise training
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DOI：
10.14814/phy2.12788
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发表时间：
2016-05-01
影响因子：
2.5
通讯作者：
Pilegaard, Henriette
中科院分区：
文献类型：
--
作者：
Knudsen, Jakob G.;Joensen, Ella;Pilegaard, Henriette
白细胞介素 (IL)-6 在运动过程中从骨骼肌 (SkM) 释放，并已被证明会影响肝脏代谢。然而，尚不清楚 SkM IL-6 是否参与调节运动训练引起的对高脂饮食 (HFD) 喂养引起的肝脏碳水化合物和脂质代谢变化的抵消作用。雄性 SkM 特异性 IL-6 KO (MKO) 和 Floxed 小鼠接受 Chow 饮食、HFD 或 HFD 结合运动训练 (HFD ExTr) 16 周。在 Floxed 小鼠中，HFD 和 HFD ExTr 均导致肝脏磷酸烯醇丙酮酸羧激酶 (PEPCK) 蛋白含量下降，但在 HFD 小鼠中，IL-6 MKO 小鼠的肝磷酸烯醇丙酮酸羧激酶 (PEPCK) 蛋白含量有所增加。此外，IL-6 MKO 小鼠的肝内葡萄糖浓度在 HFD 组中高于饮食组。在 HFD ExTr 小鼠中，IL-6 MKO 中的肝葡萄糖 6 磷酸酶 (G6Pase) 36 kDa 蛋白含量高于 Floxed 小鼠。 HFD ExTr 组中 Floxed 小鼠的肝丙酮酸脱氢酶激酶 (PDK) 4 和 PDK2 蛋白含量低于 Chow 小鼠。此外，HFD 中肝脏 ACC1 磷酸化较高，ACC1 蛋白较低。总之，这表明 SkM IL-6 调节肝脏葡萄糖代谢，但似乎对于 HFD 或 HFD 结合运动训练期间肝脏氧化能力或脂肪生成的调节并不重要。
Interleukin (IL)-6 is released from skeletal muscle (SkM) during exercise and has been shown to affect hepatic metabolism. It is, however, unknown whether SkM IL-6 is involved in the regulation of exercise training-induced counteraction of changes in carbohydrate and lipid metabolism in the liver in response to high-fat diet (HFD) feeding. Male SkM-specific IL-6 KO (MKO) and Floxed mice were subjected to Chow diet, HFD or HFD combined with exercise training (HFD ExTr) for 16 weeks. Hepatic phosphoenolpyruvate carboxykinase (PEPCK) protein content decreased with both HFD and HFD ExTr in Floxed mice, but increased in IL-6 MKO mice on HFD. In addition, the intrahepatic glucose concentration was in IL-6 MKO mice higher in HFD than chow. Within HFD ExTr mice, hepatic glucose-6-phosphatase (G6Pase) 36 kDa protein content was higher in IL-6 MKO than Floxed mice. Hepatic pyruvate dehydrogenase kinase (PDK) 4 and PDK2 protein content was in Floxed mice lower in HFD ExTr than Chow. In addition, hepatic ACC1-phosphorylation was higher and ACC1 protein lower in HFD. Together this suggests that SkM IL-6 regulates hepatic glucose metabolism, but does not seem to be of major importance for the regulation of oxidative capacity or lipogenesis in liver during HFD or HFD combined with exercise training.