S1 nuclease sensitivity to cis- and trans-diamminedichloroplatinum(II) modified DNAS: influence of (G+C) content and nucleotide sequence.
S1 nuclease sensitivity to cis- and trans-diamminedichloroplatinum(II) modified DNAS: influence of (G+C) content and nucleotide sequence.
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S1 核酸酶对顺式和反式二氯二氨铂 (II) 修饰的 DNAS 的敏感性：(GC) 含量和核苷酸序列的影响。
DOI：
10.1016/0006-291x(84)91562-6
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发表时间：
1984
影响因子：
3.1
通讯作者：
Vincent J. Capponi
中科院分区：
文献类型：
--
作者：
W. Scovell;Vincent J. Capponi
本文研究了S_1核酸酶对顺式和反式（NH_3）_2 PtCl_2修饰的DNA的敏感性，它是（1）顺式和反式（NH_3）_2 PtCl_2结合水平，（2）不同来源DNA中（G+C）含量%，（3）poly（dG）、poly（dC）和poly（dG-dC）、poly（dG-dC）序列依赖性的函数。DNA消化的程度随着任一异构体水平的增加而增加，并且受到DNA的%（G+C）含量的相反影响。然而，顺式和反式-（NH_3）_2 PtCl_2修饰的DNA在相同结合异构体水平下的消化程度的差异遵循小牛胸腺> M的顺序。多聚（dG-dC）。虽然两种异构体修饰的poly（dG-dC）·poly（dG-dC）的消化曲线几乎没有差异，但顺式和反式-（NH3）2 PtCl 2修饰的poly（dG）·poly（dC）之间的消化程度存在显著差异。这些结果进行了讨论，根据（1）的可能模式的结合顺式-（NH 3）2 PtCl 2，（2）以前报道的研究结果修饰的DNA和（3）可能的影响，在细胞染色质的修饰。
The sensitivity of S1 nuclease to cis- and trans-(NH3)2PtCl2modified DNAs is examined as a function of (1) the level of cis- and trans-(NH3)2PtCl2bound, (2) the % (G+C) content in DNA from different sources and (3) the sequence dependence in poly(dG).poly(dC) and poly(dG-dC).poly(dG-dC). The extent of DNA digested increases with increasing levels of either isomer and is inversely influenced by the % (G+C) content of the DNA. However, the difference in the extent of digestion between the cis-and trans-(NH3)2PtCl2modified DNAs at equivalent levels of bound isomer follows the order, calf-thymus > M. lysodeikticus > poly(dG-dC).poly(dG-dC). While there is virtually no difference in the digestion profiles for poly(dG-dC).poly(dG-dC) modified with the two isomers, there is a striking difference in the extent of digestion between cis- and trans-(NH3)2PtCl2modified poly(dG).poly(dC). These results are discussed in light of (1) the possible modes of binding for cis-(NH3)2PtCl2, (2) previously reported findings on modified DNA and (3) possible implications for modifications in cellular chromatin.
使用核酸外切酶 III 确定 DNA 确定序列中稳定损伤的位点：环丁烷嘧啶二聚体以及顺式和反式二氯二氨铂 II 示例。
影响因子：
14.9
作者：
Royer-Pokora,B;Gordon,LK;Haseltine,WA
通讯作者：
Haseltine,WA