The frpB1 gene of Helicobacter pylori is regulated by iron and encodes a membrane protein capable of binding haem and haemoglobin
The frpB1 gene of Helicobacter pylori is regulated by iron and encodes a membrane protein capable of binding haem and haemoglobin
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DOI：
10.1016/j.febslet.2012.02.015
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发表时间：
2012-03-23
期刊：
影响因子：
3.5
通讯作者：
de Jesus Olivares-Trejo, Jose
中科院分区：
文献类型：
--
作者：
Angel Carrizo-Chavez, Miguel;Cruz-Castaneda, Areli;de Jesus Olivares-Trejo, Jose
FrpB1是幽门螺杆菌的一种新型膜蛋白，能够结合血红素和血红蛋白，但始终对血红素表现出更强的亲和力。铁可抑制frpB1 mRNA水平，血红素或血红蛋白可轻微调节。当提供血红素或血红蛋白作为唯一铁源时，frpB1基因的过表达支持细胞生长。三维模型揭示了结合血红素或血红蛋白所必需的基序的存在。我们的总体结果支持FrpB1是幽门螺杆菌的一种膜蛋白，允许这种病原体在人胃中存活。(C) 2012年欧洲生化学会联合会。Elsevier B.V.版权所有。
FrpB1 is a novel membrane protein of Helicobacter pylori that is capable of binding both haem and haemoglobin but consistently shows more affinity for haem. The mRNA levels of frpB1 were repressed by iron and lightly modulated by haem or haemoglobin. The overexpression of the frpB1 gene supported cellular growth when haem or haemoglobin were supplied as the only iron source. Three-dimensional modelling revealed the presence of motifs necessary to bind either haem or haemoglobin. Our overall results support the idea that FrpB1 is a membrane protein of H. pylori that allows this pathogen to survive in the human stomach. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.