A coactivator of pre-mRNA splicing
A coactivator of pre-mRNA splicing
复制标题
DOI：
10.1101/gad.12.7.996
复制
发表时间：
1998-04-01
影响因子：
10.5
通讯作者：
Sharp, PA
中科院分区：
文献类型：
--
作者：
Blencowe, BJ;Issner, R;Sharp, PA
由单克隆抗体（mAb）B1 C8识别的核基质抗原是一种新的富含丝氨酸（S）和精氨酸（R）的蛋白质，与剪接复合物相关，在此被命名为SRm 160（kD的SR相关基质蛋白）。SRm 160含有多个SR重复序列，但与剪接因子的SR家族的蛋白质不同，SRm 160缺乏RNA识别基序。SRm 160和相关蛋白SRm 300（由mAb B4 A11识别的300-kD核基质抗原）形成一种复合物，该复合物是剪接特定前体mRNA所必需的。SRm 160/300复合物与剪接复合物结合，并通过与SR家族蛋白的相互作用促进剪接。SRm 160/300与前mRNA的结合通常也依赖于U1 snRNP，并由U2 snRNP稳定。因此，SRm 160/300与直接结合到前mRNA内的重要位点的组分形成多种相互作用。结果表明，核基质蛋白SRm 160和SRm 300的复合物作为前体mRNA剪接的共激活剂发挥作用。
The nuclear matrix antigen recognized by the monoclonal antibody (mAb) B1C8 is a novel serine (S) and arginine (R)-rich protein associated with splicing complexes and is named here SRm160 (SR-related matrix protein of kD). SRm160 contains multiple SR repeats, but unlike proteins of the SR family of splicing factors, lacks an RNA recognition motif. SRm160 and a related protein SRm300 (the 300-kD nuclear matrix antigen recognized by mAb B4A11) form a complex that is required for the splicing of specific pre-mRNAs. The SRm160/300 complex associates with splicing complexes and promotes splicing through interactions with SR family proteins. Binding of SRm160/300 to pre-mRNA is normally also dependent on U1 snRNP and is stabilized by U2 snRNP. Thus, SRm160/300 forms multiple interactions with components bound directly to important sites within pre-mRNA. The results suggest that a complex of the nuclear matrix proteins SRm160 and SRm300 functions as a coactivator of pre-mRNA splicing.