Overlapping CRE and E-box promoter elements can independently regulate COX-2 gene transcription in macrophages
Overlapping CRE and E-box promoter elements can independently regulate COX-2 gene transcription in macrophages
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DOI：
10.1016/s0014-5793(01)02422-x
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发表时间：
2001-05-11
期刊：
影响因子：
3.5
通讯作者：
Daly, JM
中科院分区：
文献类型：
--
作者：
Mestre, JR;Rivadeneira, DE;Daly, JM
Macrophage cyclooxygenase-2 (COX-2) transcription is mediated through the collaboration of different promoter elements. Here, the role of an overlapping cyclic AMP responsive element (CRE)/E-box was investigated. Nuclear proteins bound both the CRE and E-box, which synergized with other promoter elements to induce COX-2 transcription. Endotoxin induced binding of nuclear proteins to the CRE and E-box and each element independently induced higher COX-2 transcription levels than the overlapping CRE/E-box. Transcription factors associated with the CRE binding complex included c-Jun and CRE binding protein and with the E-box binding complex USF-1; their overexpression significantly induced COX-2 transcription. Therefore, both CRE and E-box promoter elements regulate COX-2 transcription in macrophages. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
