Gradual graft-cell repopulation with recipient cells following vascularized bone and limb allotransplantation
Gradual graft-cell repopulation with recipient cells following vascularized bone and limb allotransplantation
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DOI：
10.1002/micr.20173
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发表时间：
2005-01-01
期刊：
影响因子：
2.1
通讯作者：
Bishop, AT
中科院分区：
文献类型：
--
作者：
Muramatsu, K;Kurokawa, Y;Bishop, AT
关于血管化同种异体骨移植后移植细胞的命运知之甚少。本研究旨在确定移植细胞与受体细胞再增殖的过程。在性别不匹配的大鼠对中，进行了65例带血管蒂的胫骨和50例同种异体肢体移植。FK 506用于免疫抑制。移植物中供体和受体细胞的比例通过半定量聚合酶链反应，使用Y染色体引物进行评估。同种异体骨移植无排斥反应发生。在血管化骨同种异体移植物模型中，供体来源的细胞逐渐被受体来源的细胞取代，使得到24周时，它们仅占总细胞的10%。在肢体同种异体移植模型中，在移植后48周而不是1周时在雌性移植物中检测到雄性受体细胞。在股骨和胫骨中，受体细胞的比例大于10%。随着时间的推移，受体来源的细胞逐渐迁移到移植骨细胞中。(c)2005 Wiley-Liss，Inc.
Little is known about the fate of graft cells following vascularized bone allografting. This study was conducted to define the process of graft-cell repopulation with recipient cells. Sixty-five vascularized tibial bone and 50 limb allotransplantations were performed in rat sex-mismatched pairs. FK506 was used for immunosuppression. The ratio of donor and recipient cells in the graft was evaluated by semiquantitative polymerase chain reaction, using the Y-chromosome primers. Allografted bones had no rejection episodes. In the vascularized bone allograft model, donor-derived cells were gradually replaced by cells of recipient origin, such that by 24 weeks, they comprised only 10% of total cells. In the limb allograft model, male recipient cells were detected in female grafts not at 1 week but at 48 weeks posttransplantation. The ratio of recipient cells was more than 10% in the femur and tibia. Recipient-derived cells gradually migrated into the grafted bone cells with the passage of time. (c) 2005 Wiley-Liss, Inc.