Assembly of the cell division protein FtsZ into ladder-like structures in the aerial hyphae of Streptomyces coelicolor
Assembly of the cell division protein FtsZ into ladder-like structures in the aerial hyphae of Streptomyces coelicolor
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DOI：
10.1111/j.1365-2958.1997.mmi507.x
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发表时间：
1997-09-01
影响因子：
3.6
通讯作者：
Losick, R
中科院分区：
文献类型：
--
作者：
Schwedock, J;McCormick, JR;Losick, R
在丝状细菌天蓝色链霉菌中，细胞分裂蛋白FtsZ是将多核气生菌丝转化为单核孢子链所必需的，尽管它对于生存力并不是必需的。使用免疫荧光显微镜，我们发现FtsZ在发育中的气生菌丝中组装成长的、规则间隔的梯状阵列，平均间距约为1.3μm。在单个菌丝内，梯子的形成是相对同步的，并且延伸距离超过100μm。这些梯子仅短暂存在，随着染色体分离成不同的核仁而强度降低，并在隔膜形成完成后消失。来自免疫荧光染色总体强度的证据表明，梯子的形成部分在单个气生菌丝内 FtsZ 的积累和降解水平上受到调节。最后，FtsZ 梯子的形成受到发育控制，因为在某些所谓的白色突变体（whiG、whiH 和 whiB）中无法检测到 FtsZ 环的长阵列，这些突变体在孢子形成中受到阻碍。FtsZ 组装成梯子代表了已知的最早的分子表现形式。孢子形成过程，它的发现提供了对wh基因在气生菌丝转化为孢子链中的作用的深入了解。我们还描述了细胞壁染色技术的一种新用途，以可视化营养生长菌丝中顶端生长。
In the filamentous bacterium Streptomyces coelicolor, the cell division protein FtsZ is required for the conversion of multinucleoidal aerial hyphae into chains of uninucleoidal spores, although it is not essential for viability, Using immunofluorescence microscopy, we have shown that FtsZ assembles into long, regularly spaced, ladder-like arrays in developing aerial hyphae, with an average spacing of about 1.3 mu m. Within individual hyphae, ladder formation was relatively synchronous and extended for distances over 100 mu m. These ladders were present only transiently, decreasing in intensity as chromosomes separated into distinct nucleoids and disappearing upon the completion of septum formation. Evidence from the overall intensity of immunofluorescence staining suggested that ladder formation was regulated in part at the level of the accumulation and degradation of FtsZ within individual aerial hyphae, Finally, FtsZ ladder formation was under developmental control in that long arrays of FtsZ rings could not be detected in certain so-called white mutants (whiG, whiH and whiB), which are blocked in spore formation, The assembly of FtsZ into ladders represents the earliest known molecular manifestation of the process of spore formation, and its discovery provides insight into the role of whi genes in the conversion of aerial hyphae into chains of spores, We have also described a novel use of a cell wall-staining technique to visualize apical tip growth in vegetatively growing hyphae.