The microstructure and micromechanics of the tendon-bone insertion
The microstructure and micromechanics of the tendon-bone insertion
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DOI：
10.1038/nmat4863
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发表时间：
2017-06-01
期刊：
影响因子：
41.2
通讯作者：
Bausch, A. R.
中科院分区：
文献类型：
--
作者：
Rossetti, L.;Kuntz, L. A.;Bausch, A. R.
肌腱与骨的承重连接的特殊力学性能依赖于其生物分子组成、微观结构和微观力学的复杂相互作用。在这里，我们发现跟腱-骨止点的特征是一个类似于500 μ m的界面区域，具有独特的纤维组织和生物分子组成。在该区域内，我们通过微力学测试与多尺度共聚焦显微镜相结合，确定了异质力学响应。这导致局部应变可能大于远程应用的应变。在界面区承受大部分载荷的纤维子集随着施加力的角度而变化。蛋白质组学分析检测到22种蛋白质在界面区域富集，这些蛋白质主要参与软骨和骨骼发育以及蛋白聚糖代谢。所提出的机制为进一步合理设计软硬界面的仿生策略提供了指导。
The exceptional mechanical properties of the load-bearing connection of tendon to bone rely on an intricate interplay of its biomolecular composition, microstructure and micromechanics. Here we identify that the Achilles tendon-bone insertion is characterized by an interface region of similar to 500 mu m with a distinct fibre organization and biomolecular composition. Within this region, we identify a heterogeneous mechanical response by micromechanical testing coupled with multiscale confocal microscopy. This leads to localized strains that can be larger than the remotely applied strain. The subset of fibres that sustain the majority of loading in the interface area changes with the angle of force application. Proteomic analysis detects enrichment of 22 proteins in the interfacial region that are predominantly involved in cartilage and skeletal development as well as proteoglycan metabolism. The presented mechanisms mark a guideline for further biomimetic strategies to rationally design hard-soft interfaces.