Limited transmission of drug-resistant HIV type 1 in 100 Swedish newly detected and drug-naive patients infected with subtypes A, B, C, D, G, U, and CRF01_AE
Limited transmission of drug-resistant HIV type 1 in 100 Swedish newly detected and drug-naive patients infected with subtypes A, B, C, D, G, U, and CRF01_AE
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DOI：
10.1089/088922203322588341
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发表时间：
2003-11-01
影响因子：
1.5
通讯作者：
Leitner, T
中科院分区：
文献类型：
--
作者：
Maljkovic, I;Wilbe, K;Leitner, T
研究了新诊断的HIV-1病例中遗传耐药的患病率和潜在的亚型特异性突变模式。从瑞典三家艾滋病毒诊所随机抽取了100名新诊断患者的样本，这些样本是在1998年6月至2001年8月期间预期收集的。从血浆中提取病毒RNA，对其编码的蛋白水解酶(PR)和逆转录酶(RT)基因进行了约2000个碱基的序列测定。发现了A、B、C、D、G、U和CRF01_AE型。所有100个序列都有突变，据报道与某些耐药性有关，揭示了自然产生的亚型特异性氨基酸模式。在治疗感染非B亚型病毒的患者时，这些模式可能是重要的考虑因素。虽然许多耐药性突变似乎是自然发生的，但瑞典新发现的9%的患者可能是从接受抗病毒治疗的患者那里感染的病毒。在耐药病毒感染者中，多数为B亚型病毒感染者，属于同性恋高危人群。在新感染的病例中定期检测耐药性可能很重要，以改进治疗药物的选择，因为病毒可能会逆转，耐药形式可能会变得潜伏。
The prevalence of genetic drug resistance in newly diagnosed HIV-1 cases and potential subtype-specific mutation patterns were studied. Samples from 100 newly diagnosed patients were randomly chosen from three HIV clinics in Sweden, prospectively collected during the period June 1998 to August 2001. Viral RNA was extracted from plasma and an approximately 2000-bp fragment covering the protease (PR) and reverse transcriptase (RT) genes was sequenced. Subtypes A, B, C, D, G, U, and CRF01_AE were found. All 100 sequences had mutations reported to be involved in some drug resistance, revealing naturally occurring subtype-specific amino acid patterns. Such patterns may be important to consider when treating patients infected with nonsubtype B viruses. While many drug resistance mutations seem to be naturally occurring, 9% of the newly detected patients in Sweden may have been infected with virus from antiviral-treated patients. Among the individuals infected with resistant virus, the majority were infected with subtype B virus and belonged to the homosexual risk group. It may be important to routinely test for resistance in newly infected cases to improve the choice of drugs for treatment because the virus may revert and resistant forms can become latent.