Loss and recovery of the blood-nerve barrier in the rat sciatic nerve after crush injury are associated with expression of intercellular junctional proteins
Loss and recovery of the blood-nerve barrier in the rat sciatic nerve after crush injury are associated with expression of intercellular junctional proteins
复制标题
DOI：
10.1016/s0014-4827(02)00035-6
复制
发表时间：
2003-04-01
影响因子：
3.7
通讯作者：
Oyamada, M
中科院分区：
文献类型：
--
作者：
Hirakawa, H;Okajima, S;Oyamada, M
周围神经中的血-神经屏障对于维持轴突的环境是重要的。由神经损伤引起的屏障破坏引起各种病理。我们推测损伤后血神经屏障的破坏和恢复与细胞间连接蛋白表达的变化有关。为了验证这一假设，我们诱导挤压损伤大鼠坐骨神经结扎和claudin-1，claudin-5，occludin，VE-钙粘蛋白，和connexin 43的时空变化的免疫共聚焦显微镜和形态计量学分析，并比较他们的变化与血神经屏障的渗透性静脉和局部给药的伊文思蓝白蛋白（EBA）。结扎后第1天，EBA渗漏到神经内膜的结缔组织中，然后渗漏逐渐减少，并在第7天消失。在第1天，claudin-1、claudin-5、occludin、VE-钙粘蛋白和连接蛋白43从神经束膜和神经内膜完全消失。此后，claudin-1、claudin-5、occludin和VE-钙粘蛋白从第2天恢复，而连接蛋白43在第5天重新检测到。这些结果表明，血神经屏障的破坏和随后的恢复与claudins、occludin、VE-钙粘蛋白和连接蛋白43的表达变化密切相关，并且恢复时间过程相似但不相同。(C)2003 Elsevier Science（美国）。All rights reserved.
The blood-nerve barrier in peripheral nerves is important for maintaining the environment for axons. Breakdown of the barrier by nerve injury causes various pathologies. We hypothesized that the breakdown and recovery of the blood-nerve barrier after injury are associated with the changes in the expression of intercellular junctional proteins. To test this hypothesis, we induced crush injuries in the rat sciatic nerve by ligation and analyzed spatiotemporal changes of claudin-1, claudin-5, occludin, VE-cadherin, and connexin43 by immunoconfocal microscopy and morphometry and compared them with changes in the permeability of the blood-nerve barrier by intravenous and local administration of Evans blue-albumin (EBA). On day 1 after removal of the ligature EBA leaked into the connective tissue in the endoneurium and then the leakage gradually decreased and disappeared on day 7. On day I claudin-1, claudin-5, occludin, VE-cadherin, and connexin43 had totally disappeared from the perineurium and endoneurium. Thereafter, claudin-1, claudin-5, occludin, and VE-cadherin recovered from day 2, whereas connexin43 was redetected on day 5. These results indicate that the breakdown and following recovery of the blood-nerve barrier are closely associated with changes in the expression of claudins, occludin, VE-cadherin, and connexin43 and that the recovery time course is similar but nonidentical. (C) 2003 Elsevier Science (USA). All rights reserved.