Treatment of rabbit growth plate injuries with oriented ECM scaffold and autologous BMSCs.
Treatment of rabbit growth plate injuries with oriented ECM scaffold and autologous BMSCs.
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DOI：
10.1038/srep44140
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发表时间：
2017-03-07
影响因子：
4.6
通讯作者：
Zhao B
中科院分区：
文献类型：
--
作者：
Li W;Xu R;Huang J;Bao X;Zhao B
组织工程技术为利用生物相容性和可生物降解的支架和合适的细胞修复生长板损伤提供了一种有前途的方法。本研究的目的是制造定向ECM支架，以模仿天然生长板的材料和结构，并研究支架中的BMSCs是否可以防止受伤的生长板中骨桥的形成。我们开发了一种天然的，无细胞的和定向的支架来源于生长板。利用新的冷冻干燥技术，通过交联生长板中的微丝来制备定向支架。组织学观察显示，该支架含有GAG和胶原蛋白II等ECM成分，无细胞DNA片段;扫描电镜观察显示，该支架横向孔径均匀，纵向呈柱状结构。MTT法细胞毒性试验显示支架提取物对BMSCs无细胞毒性作用。自体骨髓基质干细胞定向支架修复兔胫骨近端生长板损伤时，可促进生长板再生，阻止骨桥形成，减少胫骨近端的角度畸形和长度不等。良好表征的ECM衍生的定向生长板支架显示出对幼兔中损伤的生长板的修复的潜力。
Tissue-engineered technology has provided a promising method for the repair of growth plate injuries using biocompatible and biodegradable scaffolds and appropriate cells. The aim of this study was to fabricate oriented ECM scaffolds to imitate the material and structure of a natural growth plate and to investigate whether BMSCs in a scaffold could prevent the formation of bone bridges in an injured growth plate. We developed a natural, acellular and oriented scaffold derived from a growth plate. The oriented scaffold was fabricated using new freeze-drying technology and by cross-linking the microfilaments in the growth plate. From histological examination, the scaffold contained most of the ECM components including GAG and collagen II without cell DNA fragments, and SEM revealed that oriented scaffold had a uniform aperture in the transverse plane and columnar structure in length plane. Cytotoxicity testing with MTT showed no cytotoxic effect of the scaffold extracts on BMSCs. Autogenous BMSCs in oriented scaffolds promoted the regeneration of neogenetic growth plate when repairing an injured growth plate and prevent the formation of bone bridges to reduce the angular deformity and length discrepancy in the proximal tibia in rabbits. The well-characterized ECM-derived oriented growth plate scaffold shows potential for the repair of injured growth plates in young rabbits.