Screening strategies and laboratory assays to support Plasmodium falciparum histidine-rich protein deletion surveillance: where we are and what is needed.
Screening strategies and laboratory assays to support Plasmodium falciparum histidine-rich protein deletion surveillance: where we are and what is needed.
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DOI：
10.1186/s12936-022-04226-2
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发表时间：
2022-06-24
期刊：
影响因子：
3
通讯作者：
Rogier, Eric
中科院分区：
文献类型：
--
作者：
Beshir, Khalid B.;Parr, Jonathan B.;Cunningham, Jane;Cheng, Qin;Rogier, Eric
关键词：
检测恶性疟原虫富组氨酸蛋白2（HRP 2）的快速诊断试验（RDTs）已成为疟疾诊断的重要工具，特别是在缺乏高质量显微镜的资源有限的环境中。目前已在亚洲、非洲和南美洲的数十个国家发现了编码该抗原的pfhrp 2基因缺失的恶性疟原虫寄生虫，新的报告显示在某些选定地区缺失的流行率很高。为了确定基于HRP 2的RDT是否适合在当地继续使用，需要对地方性恶性疟原虫种群进行重点调查和/或监测活动。各种调查和实验室方法已被用于确定寄生虫HRP 2表型和pfhrp 2基因型，这些不同的方法收集的数据需要在适当的调查和分析的背景下进行解释。HRP 2抗原的表达可以使用即时RDT或基于实验室的免疫测定来评估，但是确认pfhrp 2的缺失（或突变）需要更密集的实验室分子测定，并且对于大型调查特别需要用于严格但实用的数据收集的新工具和策略。由于疟疾诊断策略通常是在国家一级制定的，因此可能需要涵盖广泛地理区域和大量人口的具有全国代表性的调查和/或监测。本文讨论了用于恶性疟原虫HRP 2状态的表型和基因型评价的当代检测方法，考虑了它们的优点和缺点，并强调了与有效诊断政策决策所需的及时和资源意识工作流程相关的关键概念。在线版本包含补充材料，可通过10.1186/s12936-022-04226-2获得。
Rapid diagnostic tests (RDTs) detecting Plasmodium falciparum histidine-rich protein 2 (HRP2) have been an important tool for malaria diagnosis, especially in resource-limited settings lacking quality microscopy. Plasmodium falciparum parasites with deletion of the pfhrp2 gene encoding this antigen have now been identified in dozens of countries across Asia, Africa, and South America, with new reports revealing a high prevalence of deletions in some selected regions. To determine whether HRP2-based RDTs are appropriate for continued use in a locality, focused surveys and/or surveillance activities of the endemic P. falciparum population are needed. Various survey and laboratory methods have been used to determine parasite HRP2 phenotype and pfhrp2 genotype, and the data collected by these different methods need to be interpreted in the appropriate context of survey and assay utilized. Expression of the HRP2 antigen can be evaluated using point-of-care RDTs or laboratory-based immunoassays, but confirmation of a deletion (or mutation) of pfhrp2 requires more intensive laboratory molecular assays, and new tools and strategies for rigorous but practical data collection are particularly needed for large surveys. Because malaria diagnostic strategies are typically developed at the national level, nationally representative surveys and/or surveillance that encompass broad geographical areas and large populations may be required. Here is discussed contemporary assays for the phenotypic and genotypic evaluation of P. falciparum HRP2 status, consider their strengths and weaknesses, and highlight key concepts relevant to timely and resource-conscious workflows required for efficient diagnostic policy decision making. The online version contains supplementary material available at 10.1186/s12936-022-04226-2.
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影响因子：
7.4
作者：
Emslie, Kerry R.;McLaughlin, Jacob L. H.;Burke, Daniel G.
通讯作者：
Burke, Daniel G.
影响因子：
--
作者：
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通讯作者：
Ye H
影响因子：
3
作者：
Kyabayinze DJ;Tibenderana JK;Odong GW;Rwakimari JB;Counihan H
通讯作者：
Counihan H
影响因子：
4.3
作者：
Košir AB;Divieto C;Pavšič J;Pavarelli S;Dobnik D;Dreo T;Bellotti R;Sassi MP;Žel J
通讯作者：
Žel J
影响因子：
3.7
作者：
Jones, Sophie;Subramaniam, Gireesh;Talundzic, Eldin
通讯作者：
Talundzic, Eldin