The fungicidal activity of novel nanoemulsion (X8W60PC) against clinically important yeast and filamentous fungi
The fungicidal activity of novel nanoemulsion (X8W60PC) against clinically important yeast and filamentous fungi
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DOI：
10.1023/a:1021129710316
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发表时间：
2002-01-01
期刊：
影响因子：
5.5
通讯作者：
Baker, JR
中科院分区：
文献类型：
--
作者：
Myc, A;Vanhecke, T;Baker, JR
表面活性剂纳米乳是油制剂中的水，被证明具有广谱杀灭多种微生物的活性，包括革兰氏阳性和革兰氏阴性细菌、孢子和包膜病毒。这些制剂在杀生物浓度下对皮肤、粘膜和胃肠道组织无毒。在这项研究中，0.1%的纳米乳剂X8W(60)PC在15分钟内显示出对白色念珠菌和热带念珠菌等酵母菌的杀真菌活性。热带镰刀菌比白色镰刀菌更敏感，白色镰刀菌需要更长的时间和更高浓度的纳米乳才能杀灭。中性至微碱性pH比酸性pH对酵母细胞的杀伤效果更好。最低抑菌浓度试验表明，0.08%的纳米乳对白色念珠菌、假丝酵母菌、红毛菌、烟曲霉和尖孢镰刀菌等丝状真菌均有抑菌作用。在相同浓度下，单个成分的杀真菌效果都不如纳米乳。这表明纳米乳的结构是影响其抗真菌活性的重要因素。X8W(60)PC作为一种局部抗真菌药物具有很大的潜力，对其杀真菌作用机制的进一步研究是必要的。
Surfactant nanoemulsions are water in oil preparations that proved to have a broad spectrum biocidal activity against a variety of microorganisms including Gram-positive and Gram-negative bacteria, spores and enveloped viruses. These preparations are non-toxic to the skin, mucous membrane and gastrointestinal tissues at biocidal concentrations. In this study, 0.1% of the nanoemulsion designated X8W(60)PC has shown fungicidal activity against yeast including Candida albicans and C. tropicalis in 15 minutes. C. tropicalis was more sensitive than C. albicans, which required a longer time or a higher concentration of the nanoemulsion to achieve killing. Neutral to slightly alkaline pH was more effective in killing the yeast cells than acidic pH. Using the minimum inhibitory concentration assay, 0.08% of the nanoemulsion was inhibitory to C. albicans, and parapsilosis and filamentous fungi including Microsporum gypseum, Trichophyton mentagrophytes, Trichophyton rubrum, Aspergillus fumigatus and Fusarium oxysporum. None of the individual ingredients was as effective a fungicidal as the nanoemulsion at equivalent concentration. This shows that the nanoemulsion structure is an important factor in the anti-fungal activity. The X8W(60)PC has great potential as a topical anti-fungal agent and further investigation into the mechanism of fungicidal action is warranted.