The effect of surface roughness on RAW 264.7 macrophage phenotype
The effect of surface roughness on RAW 264.7 macrophage phenotype
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DOI：
10.1002/jbm.a.34562
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发表时间：
2013-09-01
影响因子：
4.9
通讯作者：
Brunette, Donald M.
中科院分区：
文献类型：
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作者：
Barth, Katrin A.;Waterfield, J. Douglas;Brunette, Donald M.
单核细胞来源的细胞，包括巨噬细胞和异物巨细胞，可以决定植入设备的性能。巨噬细胞与生物材料接触后，可被激活为典型的炎症(M1)或伤口愈合(M2)表型。此前，我们发现粗制SLA种植体上的高巨噬细胞密度与早期骨形成有关。这项研究探讨了一种可能的机制，即巨噬细胞表面粗糙度激活M2表型，以促进SLA表面的骨形成。将RAW 264.7巨噬细胞接种于SLA或光滑(Po)环氧基底物上，于培养第1、3、5天用定量聚合酶链式反应(QPCR)检测M1和M2特异性标志物NOS2和Arg-1的表达，并用抗体阵列和ELISA法检测炎症相关细胞因子和趋化因子的分泌。对照组包括用脂多糖/干扰素-1和IL-4分别刺激M1或M2表型的RAW 264.7巨噬细胞。粗糙的SLA表面不能激活Arg-1和NOS2的表达，但相对于Po表面，MCP-1和MIP-1在5天后上调，而M1相关趋化因子IP-10的分泌减少。因此，SLA表面的RAW 264.7巨噬细胞采用了M2样表型的元素，这表明当植入SLA表面时，可能会促进伤口的修复。(C)2013 Wiley期刊，Inc.生物材料资源A部分：101A：2679-2688,2013。
Monocyte-derived cells, including macrophages and foreign body giant cells, can determine the performance of implanted devices. Upon contact with biomaterials, macrophages can be activated into a classic inflammatory (M1) or wound-healing (M2) phenotype. Previously, we showed that high macrophage density on rough SLA implants was associated with early bone formation. This study examined a possible mechanism, namely, surface roughness activation of macrophages to the M2 phenotype to enhance bone formation on the SLA surface. RAW 264.7 macrophages were seeded on SLA or smooth (Po) epoxy substrates and the expression of the M1 and M2 specific markers, NOS2 and Arg-1 measured by qPCR on days 1, 3, and 5. Additionally, secretion of inflammation-associated cytokines and chemokines was studied by antibody arrays and ELISAs. Controls included RAW 264.7 macrophages primed into the M1 or M2 phenotypes by LPS/IFN- and IL-4, respectively. Rough SLA surfaces did not activate Arg-1 and NOS2 expression, but relative to Po surfaces MCP-1 and MIP-1 were upregulated after 5 days, whereas the secretion of the M1-associated chemokine IP-10 was lowered. RAW 264.7 macrophages on the SLA surface thus adopted elements of an M2-like phenotype, suggesting that when implanted the SLA surfaces may enhance wound repair. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 2679-2688, 2013.