Cloning and heterologous expression of a Methanococcus vannielii gene encoding a selenium-binding protein.
Cloning and heterologous expression of a Methanococcus vannielii gene encoding a selenium-binding protein.
复制标题
编码硒结合蛋白的范氏甲烷球菌基因的克隆和异源表达。
DOI：
10.1080/15216540400010818
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发表时间：
2004
期刊：
影响因子：
4.6
通讯作者：
Stadtman,TC
中科院分区：
文献类型：
--
作者：
Self,WilliamT;Pierce,Renee;Stadtman,TC
The activation and incorporation of selenium into selenocysteine containing selenoproteins has been well established in an Escherichia coli model system but there is little specific information concerning the transport and intracellular trafficking of selenium in biological systems in general. A selenium transport role is a possible function of a novel 42 kDa selenium‐binding protein that recently was purified from Methanococcus vannielii. The gene encoding a monomer of this protein (Sbp) has been cloned, sequenced and heterologously expressed in E. coli. The 8.8 kDa gene product contains 81 amino acids. The recombinant Sbp (rSbp) protein was shown to bind selenium from added selenite. The bound selenium appeared predominantly in dimeric and tetrameric forms of the protein. The gene encoding Sbp occurs in an operon that contains a carbonic anhydrase gene and selenocysteine‐containing formate dehydrogenase genes, suggesting possible roles in selenium‐dependent formate metabolism. IUBMB Life, 56: 501‐507, 2004
