Synchronization of circadian firing rhythms in cultured rat suprachiasmatic neurons
Synchronization of circadian firing rhythms in cultured rat suprachiasmatic neurons
复制标题
培养大鼠视交叉上神经元昼夜节律的同步
DOI：
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
K. Honma
中科院分区：
文献类型：
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作者：
T. Shirakawa;S. Honma;Y. Katsuno;H. Oguchi;K. Honma
The circadian clock in mammals is located in the suprachiasmatic nucleus (SCN) which consists of multiple oscillating neurons. Integration of the cellular oscillations is essential for the generation of a single circadian period in the SCN. By using a multielectrode dish (MED), we measured circadian firing rhythms in individual SCN neurons for more than 2 weeks continuously, and examined the involvement of synaptic communication in the synchronization of circadian rhythms. Cross‐correlation analysis of spontaneous action potentials revealed that a neuron pair was functionally connected by synapses when their circadian rhythms were synchronized. No correlation was found between the paired neurons whose circadian rhythms were not synchronized. Calcium (Ca2+)‐dependent synaptic transmission in the cellular communication was indicated by dose‐dependent lengthening of an intercellular spike interval and loss of spike correlation with a Ca2+ channel blocker. Approximately 60% of the SCN neurons in culture were immunoreactive to antibodies against γ‐aminobutyric acid (GABA) or glutamic acid decarboxylase (GAD). Spontaneous firing of all the neurons tested was either increased or decreased by bicuculline, the GABAA receptor antagonist. These findings indicate that synaptic communication plays a critical role in the synchronization of circadian rhythms in individual SCN neurons and the GABAergic transmission is involved in the synchronization mechanism.
母亲昼夜节律系统中断后胎儿昼夜节律的发展。
影响因子：
2.9
作者：
Shibata,S;Moore,RY
通讯作者：
Moore,RY
影响因子：
5.5
作者：
Chen, G;Trombley, PQ;vandenPol, AN
通讯作者：
vandenPol, AN