Role of NADH shuttle system in glucose-induced activation of mitochondrial metabolism and insulin secretion
Role of NADH shuttle system in glucose-induced activation of mitochondrial metabolism and insulin secretion
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DOI：
10.1126/science.283.5404.981
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发表时间：
1999-02-12
期刊：
影响因子：
56.9
通讯作者：
Kadowaki, T
中科院分区：
文献类型：
--
作者：
Eto, K;Tsubamoto, Y;Kadowaki, T
糖酵解和线粒体中的葡萄糖代谢对葡萄糖诱导的胰腺β细胞胰岛素分泌至关重要。一种或多种来自糖酵解而不是丙酮酸的因子似乎是产生导致胰岛素分泌的线粒体信号所必需的。糖酵解衍生的还原形式的烟酰胺腺嘌呤二核苷酸（NADH）的电子通过NADH穿梭系统转移到线粒体。通过取消NADH穿梭功能，葡萄糖诱导的NADH自发荧光，线粒体膜电位和腺苷三磷酸含量的增加减少，葡萄糖诱导的胰岛素分泌被废除。NADH穿梭明显地将糖酵解与线粒体能量代谢的活化偶联以触发胰岛素分泌。
Glucose metabolism in glycolysis and in mitochondria is pivotal to glucose-induced insulin secretion from pancreatic beta cells. One or more factors derived from glycolysis other than pyruvate appear to be required for the generation of mitochondrial signals that Lead to insulin secretion. The electrons of the glycolysis-derived reduced form of nicotinamide adenine dinucleotide (NADH) are transferred to mitochondria through the NADH shuttle system. By abolishing the NADH shuttle function, glucose-induced increases in NADH autofluorescence, mitochondrial membrane potential, and adenosine triphosphate content were reduced and glucose-induced insulin secretion was abrogated. The NADH shuttle evidently couples glycolysis with activation of mitochondrial energy metabolism to trigger insulin secretion.