Quantitative determination of cyclosporin A in whole blood and plasma by high performance liquid chromatography.
Quantitative determination of cyclosporin A in whole blood and plasma by high performance liquid chromatography.
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高效液相色谱法定量测定全血和血浆中环孢菌素A。
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
H. Shimakawa
中科院分区：
文献类型：
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作者：
N. Shibata;T. Minouchi;Y. Hayashi;T. Ono;H. Shimakawa
本文报道了一种简便、特异、灵敏的检测全血和血浆中免疫抑制剂环孢菌素A（CyA）治疗水平的方法，该方法采用高效液相色谱法，并涉及一种简单的液-液萃取过程。样品用0.1 mM葡聚糖硫酸钠盐脱聚，用正己烷萃取，并在Lichrosorb Si-60柱上进行色谱分析，在215 nm处进行UV检测。CyA和内标物（环孢菌素D）均清晰地分离为单个峰，在色谱图上未观察到干扰峰。全血和血浆中的检测限为5 ng/ml。通过重复分析评估的重现性令人满意，即批内和批间精密度的变异系数均低于4%。添加的CyA的分析回收率几乎完全。该方法灵敏度高，可用于肾移植患者全血和血浆中CyA浓度的监测，也可用于肾移植患者血浆和非血浆中CyA浓度分布的研究。
A simple, specific and sensitive method for monitoring therapeutic levels of the immunosuppressive agent cyclosporin A (CyA) in whole blood and plasma has been developed which uses a high performance liquid chromatography and involves a simple liquid-liquid extraction procedure. Samples were delipolysed with 0.1 mM dextran sulfate sodium salt, extracted with n-hexane, and chromatographed on a Lichrosorb Si-60 column with UV detection at 215 nm. Each of CyA and internal standard (cyclosporin D) is resoluted clearly as a single peak and no interfering peaks are observed on the chromatograms. The detection limit is 5 ng/ml in whole blood and plasma. The reproducibility assessed by repeated analysis is satisfactory, that is to say, each coefficient of variation of the within-run and between-run precision is below 4%. The analytical recovery of added CyA is almost complete. This method is sensitive enough for monitoring whole blood and plasma levels of CyA in renal transplant patients in the therapeutic dose range, and is also applicable to the study on the distribution of CyA between plasma and non-plasma fractions of blood in patients with renal allografts.