Metagenomic analysis reveals diverse polyketide synthase gene clusters in microorganisms associated with the marine sponge Discodermia dissoluta
Metagenomic analysis reveals diverse polyketide synthase gene clusters in microorganisms associated with the marine sponge Discodermia dissoluta
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DOI：
10.1128/aem.71.8.4840-4849.2005
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发表时间：
2005-08-01
影响因子：
4.4
通讯作者：
Hutchinson, CR
中科院分区：
文献类型：
--
作者：
Schirmer, A;Gadkari, R;Hutchinson, CR
海绵相关细菌被认为产生许多新的生物活性化合物，包括聚酮化合物。从海洋海绵Discodermia dissoluta的微生物群落的I型模块化聚酮酶（PKS）的酮合酶结构域的PCR扩增揭示了巨大的多样性和一组新的海绵特异性PKS酮合酶结构域。筛选了与该海绵相关的总计超过4千兆碱基的细菌基因组的宏基因组文库的I型模块化PKS基因簇。总海绵DNA文库中超过90%的克隆代表细菌DNA插入，0.7%含有PKS基因。大多数的PKS杂交克隆进行小PKS集群的一至三个模块，虽然一些克隆编码大的多模块PKS（超过五个模块）。最丰富的大型模块化PKS似乎是由细菌共生体编码的，
Sponge-associated bacteria are thought to produce many novel bioactive compounds, including polyketides. PCR amplification of ketosynthase domains of type I modular polyketide synthases (PKS) from the microbial community of the marine sponge Discodermia dissoluta revealed great diversity and a novel group of sponge-specific PKS ketosynthase domains. Metagenomic libraries totaling more than four gigabases of bacterial genomes associated with this sponge were screened for type I modular PKS gene clusters. More than 90% of the clones in total sponge DNA libraries represented bacterial DNA inserts, and 0.7% harbored PKS genes. The majority of the PKS hybridizing clones carried small PKS clusters of one to three modules, although some clones encoded large multimodular PKSs (more than five modules). The most abundant large modular PKS appeared to be encoded by a bacterial symbiont that made up