Impaired T cell function in RANTES-deficient mice
Impaired T cell function in RANTES-deficient mice
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DOI：
10.1006/clim.2001.5178
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发表时间：
2002-03-01
影响因子：
8.6
通讯作者：
Danoff, TM
中科院分区：
文献类型：
--
作者：
Makino, Y;Cook, DN;Danoff, TM
趋化因子RANTES是单核细胞和T细胞的化学引诱物，并且被假定参与免疫应答的许多方面。为了评估RANTES在体内的生物学作用，我们产生了RANTES缺陷（-/-）小鼠，并表征了它们的T细胞功能。在皮肤迟发型超敏反应试验中，与+/+小鼠相比，在-/-小鼠中观察到耳和足垫肿胀减少50%。在体外，多克隆和抗原特异性T细胞增殖减少。使用荧光染料羧基-荧光素琥珀酰亚胺酯的定量分析显示，这种增殖缺陷是由于抗原反应性T细胞减少和这些细胞增殖能力降低。此外，-/- T细胞产生的IFN-γ和IL-2显著降低。总之，这些数据表明RANTES是正常T细胞功能以及将单核细胞和T细胞募集到炎症部位所必需的。(C)2002 Elsevier Science（美国）。
The chemokine RANTES is a chemoattractant for monocytes and T cells and is postulated to participate in many aspects of the immune response. To evaluate the biological roles of RANTES in vivo, we generated RANTES-deficient (-/-) mice and characterized their T cell function. In cutaneous delayed-type hypersensitivity assays, a 50% reduction in ear and footpad swelling was seen in -/- mice compared to +/+ mice. In vitro, polyclonal and antigen-specific T cell proliferation was decreased. Quantitative analysis using the fluorescent dye carboxy-fluorescein succinimidyl ester revealed that this proliferative defect was due both to fewer antigen-reactive T cells and to a reduction in the capacity of these cells to proliferate. In addition, IFN-gamma and IL-2 production by the -/- T cells was dramatically decreased. Together, these data suggest that RANTES is required for normal T cell functions as well as for recruiting monocytes and T cells to sites of inflammation. (C) 2002 Elsevier Science (USA).