Studies on in vitro growth and pathogenicity of European Fusarium fungi
Studies on in vitro growth and pathogenicity of European Fusarium fungi
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DOI：
10.1023/a:1024712415326
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发表时间：
2003-07-01
影响因子：
1.8
通讯作者：
Doohan, FM
中科院分区：
文献类型：
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作者：
Brennan, JM;Fagan, B;Doohan, FM
研究了温度对由禾谷镰刀菌、小麦赤霉菌、燕麦镰刀菌、玉米赤霉菌和雪腐镰刀菌组成的欧洲镰刀菌的体外生长速率和致病力的影响。不分地理来源，禾谷镰刀菌、顶芽镰刀菌和玉米赤霉菌的最适生长温度为25℃，燕麦和雪莲的最适生长温度为20℃。总体而言，禾本科牧草、豆科牧草和豆科牧草的生长速度在10~25℃之间增加，燕麦和雪莲的生长速度在10~20℃之间增加。通过测定5种植物对小麦幼苗胚芽鞘生长速率的影响，进行了致病力测定。福斯塔夫)。无论地理来源如何，燕麦、燕麦和禾本科牧草对胚芽鞘生长的影响最大，温度范围为20-25℃(减幅为89.3%)，而小麦胚芽鞘和雪莲的生长延缓温度为10-15℃(减幅为45.6%)。总体而言，F culmorum和F graminearum是这五种植物中致病性最强的，在10、15、20和25℃的温度下，芽鞘生长至少减少69%。一般线性模型分析(GLIM)表明，物种对菌株生长变异的贡献率为51.3%~63.4%，对体外致病性变异的贡献率为19.5%~44.3%。原产地对生长率变异的贡献率为22.6%~51.9%，对致病性变异的贡献率为0.73%~7.61%。在体外生长和致病性之间唯一显著的相关性是在15℃下观察到的雪花分枝杆菌(r=-0.803，P<；0.05)。
The effect of temperature on the in vitro growth rates and pathogenicity of a European Fusarium collection consisting of isolates of Fusarium graminearum, F culmorum, E avenaceum, F poae and Microdochium nivale was examined. Irrespective of geographic origin, the optimum temperature for the growth of F graminearum, F culmorum and F poae was 25 degreesC, while that for E avenaceum and M. nivale was 20degreesC. In general, the growth rates of E graminearum, E culmorum and E poae increased between 10 and 25 degreesC and those of E avenaceum and M. nivale increased between 10 and 20 degreesC. Pathogenicity tests were carried out by examining the effect of the five species on the in vitro coleoptile growth rate of wheat seedlings (cv. Falstaff). Irrespective of geographic origin, the temperature at which F avenaceum, F culmorum and F graminearum caused the greatest retardation in coleoptile growth ranges 20-25 degreesC (> 89.3% reduction), whilst for E poae and M. nivale it was 10-15 degreesC (>45.6% retardation), relative to uninoculated control seedlings. In general, F culmorum and F graminearum were the most pathogenic of the five species, causing at least a 69% reduction in coleoptile growth at 10, 15, 20 and 25 degreesC. General linear model analysis (GLIM) showed that species accounted for 51.3-63.4% of the variation in isolate growth and from 19.5% to 44.3% of the variation in in vitro pathogenicity. Country of origin contributed from 22.6% to 51.9% to growth rate variation and from 0.73% to 7.61% to pathogenicity variation. The only significant correlation between in vitro growth and pathogenicity was that observed for M. nivale at 15 degreesC (r = -0.803, P < 0.05).