CONTRACTILE EFFECT OF ENDOTHELIN IN THE HUMAN UTERINE ARTERY AND AUTORADIOGRAPHIC LOCALIZATION OF ITS BINDING-SITES
CONTRACTILE EFFECT OF ENDOTHELIN IN THE HUMAN UTERINE ARTERY AND AUTORADIOGRAPHIC LOCALIZATION OF ITS BINDING-SITES
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DOI：
10.1016/s0002-9378(11)91582-8
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发表时间：
1992-09-01
影响因子：
9.8
通讯作者：
STJERNQUIST, M
中科院分区：
文献类型：
--
作者：
BODELSSON, G;SJOBERG, NO;STJERNQUIST, M
目的：研究内皮素-1在人子宫动脉中的作用、作用方式及结合部位。研究设计：体外观察内皮素-1对有、无内皮人子宫动脉的收缩作用及维拉帕米和尼卡地平对其收缩的影响。采用学生t检验。用放射自显影技术定位125I-ET-1结合部位。结果：ET-1引起的收缩不受内皮去除的影响。维拉帕米拮抗收缩，尼卡地平则无作用。结论：这些结果表明，ET-1是人子宫动脉的一种血管收缩因子，其作用机制可能是通过平滑肌细胞上的受体来实现的。这种作用方式似乎涉及二氢吡啶敏感的钙通道以外的钙离子内流，而内皮素-1似乎不能刺激其他内皮衍生的血管活性物质的释放。
OBJECTIVE: The aim was to study the effects, mode of action, and binding sites of endothelin-1 in the human uterine artery.STUDY DESIGN: The contractile effect of endothelin-1 on the human uterine artery with and without endothelium and the effect of verapamil and nicardipine on the contraction was investigated in vitro. The Student t test was used. Iodine 125-endothelin-1 binding sites were localized with autoradiography.RESULTS: Endothelin-1 induced a contraction that was unaffected by removal of the endothelium. Verapamil antagonized the contraction, whereas nicardipine showed no effect. Iodine 125-endothelin-1 binding sites were demonstrated in the smooth muscle layer.CONCLUSIONS: These results show that endothelin-1 is a vasoconstrictor in the human uterine artery and suggest that the effect is mediated by receptors on the smooth muscle cells. The mode of action seems to involve Ca++ influx by other than dihydropyridine-sensitive Ca" channels. Endothelin-1 does not seem to stimulate release of other endothelium-derived vasoactive agents.