High expression of the breast cancer susceptibility gene BRCA1 in long-lived termite kings.
High expression of the breast cancer susceptibility gene BRCA1 in long-lived termite kings.
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DOI：
10.18632/aging.101578
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发表时间：
2018-10-11
期刊：
影响因子：
--
通讯作者：
Iuchi Y
中科院分区：
文献类型：
--
作者：
Tasaki E;Mitaka Y;Nozaki T;Kobayashi K;Matsuura K;Iuchi Y
衰老与DNA损伤的积累有关。 DNA 修复基因的高表达被认为有助于延长多种生物体的寿命。然而，有助于长寿的关键 DNA 修复基因仍然未知。尽管白蚁王来自相同的基因组，但与工蚁等非繁殖个体相比，白蚁王的寿命却非常长，从而为识别长寿相关基因提供了一个独特的模型。在这项研究中，我们证明白蚁王比其他种姓表达更高水平的乳腺癌易感基因 BRCA1。通过 RNA 测序，我们在散白蚁 (Reticulitermes speratus) 的 127 个新注释的 DNA 修复基因中鉴定出了 21 个王特异性基因。通过定量PCR，我们发现一些高表达的王特异性基因在生殖组织（睾丸）中的表达与在体细胞组织（脂肪体）中的表达相比显着上调。值得注意的是，国王脂肪体内的 BRCA1 基因表达量比工蜂高 4 倍多。这些结果表明，BRCA1 部分有助于白蚁王体细胞和生殖组织的 DNA 修复。这些发现为了解 BRCA1 基因表达与白蚁王非凡寿命之间的联系提供了重要见解。
Aging is associated with the accumulation of DNA damage. High expression of DNA repair genes has been suggested to contribute to prolonged lifespan in several organisms. However, the crucial DNA repair genes contributing to longevity remain unknown. Termite kings have an extraordinary long lifespan compared with that of non-reproductive individuals such as workers despite being derived from the same genome, thus providing a singular model for identifying longevity-related genes. In this study, we demonstrated that termite kings express higher levels of the breast cancer susceptibility gene BRCA1 than other castes. Using RNA sequencing, we identified 21 king-specific genes among 127 newly annotated DNA repair genes in the termite Reticulitermes speratus. Using quantitative PCR, we revealed that some of the highly expressed king-specific genes were significantly upregulated in reproductive tissue (testis) compared to their expression in somatic tissue (fat body). Notably, BRCA1 gene expression in the fat body was more than 4-fold higher in kings than in workers. These results suggest that BRCA1 partly contributes to DNA repair in somatic and reproductive tissues in termite kings. These findings provide important insights into the linkage between BRCA1 gene expression and the extraordinary lifespan of termite kings.
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DOI：
10.1083/jcb.201110008
发表时间：
2012-04-16
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kawai S;Amano A
通讯作者：
Amano A
DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
3.7
作者：
Hayward A;Gillooly JF
通讯作者：
Gillooly JF
影响因子：
16
作者：
Ciccia A;Elledge SJ
通讯作者：
Elledge SJ