Alteration of DNA methylation level and pattern in sorghum (Sorghum bicolor L.) pure-lines and inter-line F1 hybrids following low-dose laser irradiation.
Alteration of DNA methylation level and pattern in sorghum (Sorghum bicolor L.) pure-lines and inter-line F1 hybrids following low-dose laser irradiation.
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DOI：
10.1016/j.jphotobiol.2010.03.011
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发表时间：
2010-06
期刊：
影响因子：
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通讯作者：
Huan Wang;Q. Feng;Meishan Zhang;Chunwu Yang;W. Sha;Bao Liu
中科院分区：
文献类型：
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作者：
Huan Wang;Q. Feng;Meishan Zhang;Chunwu Yang;W. Sha;Bao Liu
低剂量激光辐照可以刺激许多生物学过程，并已被广泛应用于各个领域，包括在作物改良中产生有用的突变体。尽管如此，低剂量激光辐照致突变特性的分子和遗传基础尚未阐明。DNA胞嘧啶甲基化是敏感的，并响应于内在扰动和环境线索。本研究旨在探讨低剂量激光辐照对高粱纯系和种内杂种F1 DNA甲基化稳定性的可能影响。为此，选择了一对两色高粱（Soraedbicolor L.）利用正反交F_1及其亲本纯系，用低剂量激光照射其萌发种子。采用甲基化敏感扩增多态性（MSAP）技术分析了这些植物的DNA甲基化水平和模式。结果表明，低剂量激光辐照诱导高粱植株DNA甲基化水平和模式发生低频但显著的改变，表明该处理对植物具有表观遗传诱变作用。此外，我们观察到，在F1代的线间杂种的变化频率高于其纯系亲本，这表明杂交和激光照射的交互作用。我们建议，种内杂交和表观遗传诱变处理，如低剂量激光照射的组合使用可能是一个有用的手段，在植物中产生可遗传的表观遗传变异。
Low-dose laser irradiation can stimulate a number of biological processes and has been widely used in various fields including producing useful mutants in crop improvement. Nonetheless, the molecular and genetic basis for the mutagenic property of low-dose laser irradiation has not been elucidated. DNA cytosine methylation is sensitive and responsive to both intrinsic perturbations and environmental cues. This study was aimed to probe the possible effect of low-dose laser irradiation on stability of DNA methylation in sorghum pure-lines and intraspecific F1 hybrids. For this purpose, a pair of Sorghum bicolor L. reciprocal F1 hybrids and their parental pure-lines was used, and their germinating seeds were treated by low-dose laser irradiation. The level and pattern of DNA methylation in the plants were analyzed by methylation-sensitive amplified polymorphism (MSAP). Results showed that low-dose laser irradiation induced low-frequency but significant alterations in DNA methylation level and pattern in sorghum plants, demonstrating the treatment is epigenetically mutagenic in plants. In addition, we observed that the alteration frequency in the inter-line F1 hybrids was higher than that of their pure-line parents, suggesting an interaction of hybridity and the laser irradiation. We propose that the combined use of intraspecific hybridization and an epigenetically mutagenic treatment like low-dose laser irradiation might be a useful means to generate heritable epigenetic variations in plants.