Time-course responses of ileal and fecal microbiota and metabolite profiles to antibiotics in cannulated pigs
Time-course responses of ileal and fecal microbiota and metabolite profiles to antibiotics in cannulated pigs
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插管猪回肠和粪便微生物群和代谢物谱对抗生素的时间=过程反应
DOI：
10.1007/s00253-018-8774-2
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发表时间：
2018-03-01
影响因子：
5
通讯作者：
Zhu, Wei-Yun
中科院分区：
文献类型：
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作者：
Gao, Kan;Pi, Yu;Zhu, Wei-Yun
我们在回肠插管猪模型上研究了治疗性抗生素对肠道微生物组成和代谢的时程影响。将16头体重为12+/-0.5 kg的回肠内置仔猪随机分为2组(n=8)，分别饲喂添加或不添加抗生素的标准饲料。分别于给药前4天、给药后第2、7、13天采集回肠和粪便标本，通过16S rRNA MiSeq测序和代谢产物(短链脂肪酸、生物胺和吲哚)分析微生物区系组成。结果表明，回肠中乳杆菌和双歧杆菌在第2-13天平均减少2.68倍和508倍，在第7-13天在粪便中平均减少45.08倍和71.50倍(P<；0.05)。肠杆菌/志贺氏菌在第2-13天回肠中平均增加265倍，在第7-13天在粪便中平均增加36.70倍(P<；0.05)。回肠醋酸盐浓度在第2-13天平均下降2.88倍，在第7天粪便浓度下降1.83倍(P<；0.05)。身体在回肠中的浓度在第2-13天平均增加了7.03倍，在粪便中的浓度在第7-13天平均增加了9.96倍(P<；0.05)，粪便中吲哚的浓度在第7-13天平均增加了2.51倍(P<；0.05)。显著微生物与代谢产物的相关性分析表明，抗生素诱导的微生物区系转移似乎导致了肠道代谢的变化。综上所述，抗生素的使用导致了回肠和粪便微生物群落和代谢的动态变化，回肠微生物区系比粪便微生物区系更容易发生转移。
We investigated the time-course effects of therapeutic antibiotics on intestinal microbial composition and metabolism in an ileal-cannulated pig model. Sixteen ileal-cannulated piglets (12 +/- 0.5 kg) were assigned to two groups (n = 8) and fed standard diets with or without antibiotics. At 4 days before, and at days 2, 7, and 13 after antibiotic administration, ileal and fecal samples were collected for analysis of microbiota composition via 16S rRNA MiSeq sequencing and metabolites (short-chain fatty acids, biogenic amines, and indole). It was found that Lactobacillus and Bifidobacterium had decreased by an average 2.68-fold and 508-fold in ileum on days 2-13, and by an average 45.08-fold and 71.50-fold in feces on days 7-13 (P < 0.05). Escherichia/Shigella had increased by an average 265-fold in ileum on days 2-13, and by an average 36.70-fold in feces on days 7-13 (P < 0.05). Acetate concentration had decreased in ileum by an average 2.88-fold on days 2-13, and by 1.83-fold in feces on day 7 (P < 0.05). Cadaverine concentration had increased by an average 7.03-fold in ileum on days 2-13, and by an average 9.96-fold in feces on days 7-13 (P < 0.05), and fecal indole concentration had increased by an average 2.51-fold on days 7-13 (P < 0.05). Correlation analysis between significant microbes and metabolites indicated that the antibiotic-induced microbiota shift appeared to result in the changes of intestinal metabolism. In conclusion, antibiotic administration led to dynamic changes in microbial communities and metabolism in ileum and feces, with ileal microbiota being more prone to shift than fecal microbiota.