Efficient very-high-gravity fermentation of sugarcane molasses by industrial yeast strains
Efficient very-high-gravity fermentation of sugarcane molasses by industrial yeast strains
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DOI：
10.1002/jib.317
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发表时间：
2016-06-01
影响因子：
2.6
通讯作者：
Ernandes, Jose Roberto
中科院分区：
文献类型：
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作者：
Barbosa, Heloisy Suzes;Silveira, Erick de Abreu;Ernandes, Jose Roberto
在这项研究中，工业菌株接种，在连续的周期中，在高细胞密度的甘蔗为基础的果汁含有正常（22%，w/v）和非常高的蔗糖（30%，w/v）水平，并补充蛋白胨作为氮源。在30 ° C下，在振荡培养物中，在正常重力情况下，在补充和未补充的培养基中均发生有效的蔗糖利用。在较高的糖浓度，补充蛋白胨诱导更有效的发酵相比，未补充的培养基，具有较高的生物量积累和细胞活力的维持，乙醇水平高达16%（v/v）。海藻糖在循环期间也很高，可能是酵母对高应力发酵条件的必要响应。这表明有可能通过使用超高发酵技术来增加乙醇产量，并且营养补充将对发酵产生积极影响，从而允许有效的糖消耗和细胞活力维持。版权所有（c）2016酿酒和蒸馏研究所
In this study industrial strains were inoculated, in successive cycles, at high cell density into a sugarcane-based juice containing normal (22%, w/v) and very high sucrose (30%, w/v) levels and supplemented with peptone as a nitrogen source. At 30 degrees C, in shaken cultures, in the normal gravity situation, efficient sucrose utilization occurred in both the supplemented and unsupplemented medium. At higher sugar concentrations, supplementation with peptone induced a more efficient fermentation compared with the unsupplemented medium, with higher biomass accumulation and maintenance of cell viability, and ethanol levels as high as 16% (v/v). Trehalose was also high during the cycles, probably as a necessary response of the yeast to the high stress fermentation conditions. This suggests that it is possible to increase ethanol production by using very-high-fermentation technology and that nutritional supplementation would have a positive effect on the fermentation, allowing for efficient sugar consumption and cell viability maintenance. Copyright (c) 2016 The Institute of Brewing & Distilling