RELATION BETWEEN WORK AND PHOSPHATE METABOLITE IN THE INVIVO PACED MAMMALIAN HEART
RELATION BETWEEN WORK AND PHOSPHATE METABOLITE IN THE INVIVO PACED MAMMALIAN HEART
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DOI：
10.1126/science.3704638
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发表时间：
1986-05-30
期刊：
影响因子：
56.9
通讯作者：
BRIGGS, RW
中科院分区：
文献类型：
--
作者：
BALABAN, RS;KANTOR, HL;BRIGGS, RW
核磁共振（NMR）光谱被用来监测，在一个节拍到节拍的基础上，磷酸肌酸和腺苷三磷酸在体内犬心脏的工作输出的变化过程中的浓度。在广泛的心率-血压乘积（5，000至25，000 mmHg/min）范围内，心脏内磷酸肌酸和三磷酸腺苷的相对量保持恒定。在肌酸激酶催化反应在该组织中接近平衡的合理假设下，计算游离二磷酸腺苷的相对浓度。腺苷二磷酸的游离浓度也没有改变在这个范围内的速率-压力产品。数据表明，这些化合物的浓度在体内受到高度调节，并表明除其浓度外的其他因素可能参与调节稳态心肌功输出与氧消耗。
Nuclear magnetic resonance (NMR) spectroscopy was used to monitor, on a beat-to-beat basis, the concentration of creatine phosphate and adenosine triphosphate during alterations in the work output of canine hearts in vivo. Over a wide range of rate-pressure products (5,000 to 25,000 mmHg/min), the relative amounts of creatine phosphate and adenosine triphosphate within the heart remained constant. The relative concentration of free adenosine diphosphate was calculated under the reasonable assumption that the creatine kinase-catalyzed reaction is near equilibrium in this tissue. The free concentration of adenosine diphosphate also did not change over this range of rate-pressure products. The data demonstrate that the concentration of these compounds is highly regulated in vivo and suggest that factors other than their concentration may be involved in the modulation of steady-state myocardial work output with oxygen consumption.