Cd-induced oxidative stress and lignification in the roots of two Vicia sativa L. varieties with different Cd tolerances
Cd-induced oxidative stress and lignification in the roots of two Vicia sativa L. varieties with different Cd tolerances
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DOI：
10.1016/j.jhazmat.2015.08.052
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发表时间：
2016-01-15
影响因子：
13.6
通讯作者：
Zhang, Fenqin
中科院分区：
文献类型：
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作者：
Rui, Haiyun;Chen, Chen;Zhang, Fenqin
研究了Cd对两个品种油菜根系生长、脂质过氧化、活性氧积累、抗氧化酶活性和木质素含量的影响。Cd处理对Cd敏感的品种ZM比Cd耐受的品种L3更严重地降低了植株生长，增加了ROS和脂质过氧化水平。Cd处理后，大部分过氧化氢（H2O2）和超氧阴离子（O-2）在细胞壁和胞外空间积累。化学分析和使用抑制剂的实验表明，ZM中H2O2和O-2（中心点-）的产生比13中的增加更大，可能是由于cd诱导的烟酰胺腺嘌呤二核苷酸过氧化物酶（NADH-POD）活性升高。Cd处理显著提高了ZM根外质体木质素积累和愈创木酚过氧化物酶（GPOD）活性。但13处理的根漆酶活性高于ZM处理。因此，Cd毒性诱导了苜蓿根系显著的木质化，H2O2积累和外胞体GPOD活性的增加可能是造成这一效应的原因。(C) 2015 Elsevier B.V.版权所有
We examined the effects of Cd on growth, lipid peroxidation, reactive oxygen species (ROS) accumulation, antioxidant enzymatic activity, and lignin content in the roots of two varieties of Vicia sativa. Treatment with Cd decreased plant growth and increased ROS and lipid peroxidation levels to a greater extent in the Cd-sensitive variety ZM than in the Cd-tolerant variety L3. Most hydrogen peroxide (H2O2) and superoxide anion (O-2(center dot-)) were accumulated in the cell walls and extracellular spaces in response to Cd treatments. Chemical assays and experiments using inhibitors showed that larger increases in H2O2 and O-2(center dot-) production in ZM than in 13 were probably attributed to elevated Cd-induced nicotinamide adenine dinucleotide-peroxidase (NADH-POD) activity. Cd treatment increased the accumulation of lignin and the guaiacol peroxidase (GPOD) activities in the apoplast more significantly in ZM root than in 13. Howerver, root laccase activity was higher in 13 than in ZM. Thus Cd toxicity induced significant lignification in the roots of V. sativa, and increases in H2O2 accumulation and apoplastic GPOD activity were likely responsible for this effect. (C) 2015 Elsevier B.V. All rights reserved.