Blimp1 regulates development of the posterior forelimb, caudal pharyngeal arches, heart and sensory vibrissae in mice.
Blimp1 regulates development of the posterior forelimb, caudal pharyngeal arches, heart and sensory vibrissae in mice.
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DOI：
10.1242/dev.012047
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发表时间：
2007-12
期刊：
影响因子：
--
通讯作者：
Bikoff EK
中科院分区：
文献类型：
--
作者：
Robertson EJ;Charatsi I;Joyner CJ;Koonce CH;Morgan M;Islam A;Paterson C;Lejsek E;Arnold SJ;Kallies A;Nutt SL;Bikoff EK
锌指转录抑制因子Blimp 1（Prdm 1）控制B淋巴细胞分化过程中的基因表达模式，并调节原始生殖细胞特化所需的表观遗传变化。Blimp 1在发育中的小鼠胚胎的不同组织部位动态表达，但其功能作用仍然未知，因为Blimp 1突变胚胎由于胎盘功能不全而在E10.5时停滞。为了探索Blimp 1在胚胎后期的活动，我们使用了条件失活策略。还利用Blimp 1-Cre转基因菌株来生成Blimp 1表达细胞的命运图。Blimp 1在前肢后部、咽弓尾部、次级心脏区域和感觉触须中的多能祖细胞群中起着重要作用，并在这些不同的组织部位维持关键的信号中心。有趣的是，携带亚型Blimp 1 gfp报告基因等位基因的胚胎存活至妊娠晚期，并表现出类似但不太严重的发育异常，而表达水平进一步降低的transheterozygous Blimp 1 gfpl-胚胎显示出加剧的缺陷。总的来说，本实验表明，Blimp 1在不同细胞类型中的需求是精确的剂量依赖性。
The zinc-finger transcriptional repressor Blimp1 (Prdm1) controls gene expression patterns during differentiation of B lymphocytes and regulates epigenetic changes required for specification of primordial germ cells. Blimp1 is dynamically expressed at diverse tissue sites in the developing mouse embryo, but its functional role remains unknown because Blimp1 mutant embryos arrest at E10.5 due to placental insufficiency. To explore Blimp1 activities at later stages in the embryo proper, here we used a conditional inactivation strategy. A Blimp1-Cre transgenic strain was also exploited to generate a fate map of Blimp1-expressing cells. Blimp1 plays essential roles in multipotent progenitor cell populations in the posterior forelimb, caudal pharyngeal arches, secondary heart field and sensory vibrissae and maintains key signalling centres at these diverse tissues sites. Interestingly, embryos carrying a hypomorphic Blimp1 gfp reporter allele survive to late gestation and exhibit similar, but less severe developmental abnormalities, whereas transheterozygous Blimp1 gfpl– embryos with further reduced expression levels, display exacerbated defects. Collectively, the present experiments demonstrate that Blimp1 requirements in diverse cell types are exquisitely dose dependent.
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影响因子：
2.7
作者：
Arnold SJ;Maretto S;Islam A;Bikoff EK;Robertson EJ
通讯作者：
Robertson EJ
影响因子：
30.8
作者：
Khokha, MK;Hsu, D;Harland, RM
通讯作者：
Harland, RM
影响因子：
21.3
作者：
Ancelin, Katia;Lange, Ulrike C.;Surani, M. Azim
通讯作者：
Surani, M. Azim
DOI：
10.1084/jem.20040973
发表时间：
2004-10-18
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Kallies A;Hasbold J;Tarlinton DM;Dietrich W;Corcoran LM;Hodgkin PD;Nutt SL
通讯作者：
Nutt SL
影响因子：
2.4
作者：
Graham, A
通讯作者：
Graham, A