α-Catenin stabilises Cadherin-Catenin complexes and modulates actomyosin dynamics to allow pulsatile apical contraction
α-Catenin stabilises Cadherin-Catenin complexes and modulates actomyosin dynamics to allow pulsatile apical contraction
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DOI：
10.1242/jcs.193268
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发表时间：
2016-12-15
影响因子：
4
通讯作者：
Gorfinkiel, Nicole
中科院分区：
文献类型：
--
作者：
Jurado, Jaime;de Navascues, Joaquin;Gorfinkiel, Nicole
我们已经研究了细胞收缩性和粘附性在上皮形态形成过程中是如何在功能上整合的。为此，我们通过研究一个新发现的等位基因系列，分析了α - catenin在果蝇胚胎背闭合过程中的作用。α - catenin是连接e -钙粘蛋白黏附和肌动球蛋白细胞骨架的关键分子。我们发现-连环蛋白调节羊膜的搏动性顶端收缩，羊膜是胚胎表皮闭合的主要动力组织。α - catenin通过稳定和促进肌动球蛋白焦点的形成来控制肌动球蛋白动力学，同时也稳定细胞膜上的DE-Cadherin (Drosophila E-Cadherin，也称为Shotgun)，这表明中尖肌动球蛋白的收缩性调节连接处的稳定性。此外，我们发现了α - catenin和Vinculin之间的遗传相互作用，以及Vinculin在羊膜顶端细胞膜上的张力依赖性募集，这表明在该组织中存在机械敏感模块。
We have investigated how cell contractility and adhesion are functionally integrated during epithelial morphogenesis. To this end, we have analysed the role of alpha-Catenin, a key molecule linking E-Cadherin-based adhesion and the actomyosin cytoskeleton, during Drosophila embryonic dorsal closure, by studying a newly developed allelic series. We find that alpha-Catenin regulates pulsatile apical contraction in the amnioserosa, the main force-generating tissue driving closure of the embryonic epidermis. alpha-Catenin controls actomyosin dynamics by stabilising and promoting the formation of actomyosin foci, and also stabilises DE-Cadherin (Drosophila E-Cadherin, also known as Shotgun) at the cell membrane, suggesting that medioapical actomyosin contractility regulates junction stability. Furthermore, we uncover a genetic interaction between alpha-Catenin and Vinculin, and a tension-dependent recruitment of Vinculin to amniosersoa apical cell membranes, suggesting the existence of a mechano-sensitive module operating in this tissue.