Comparative Analysis of Anther Transcriptome Profiles of Two Different Rice Male Sterile Lines Genotypes under Cold Stress
Comparative Analysis of Anther Transcriptome Profiles of Two Different Rice Male Sterile Lines Genotypes under Cold Stress
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DOI：
10.3390/ijms160511398
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发表时间：
2015-05-01
影响因子：
5.6
通讯作者：
Deng, Qi-Yun
中科院分区：
文献类型：
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作者：
Bai, Bin;Wu, Jun;Deng, Qi-Yun
水稻在生殖发育过程中对冷胁迫高度敏感，而水稻花药冷胁迫反应的机制尚不清楚。利用HiSeq 2000测序平台，对水稻光温敏核不育系Y 58 S和P64 S（培矮64 S）在育性敏感期低温胁迫下的花药转录组进行分析。从4个文库中获得约2.43亿个干净的读段，并与籼稻基因组进行比对，在P64 S和Y 58 S中分别鉴定出1497和5652个差异表达基因（DEG）。对这些DEG进行基因本体（GO）和京都基因和基因组百科全书（KEGG）分析。对DEG进行了功能分类。两种基因型共有的DEG主要参与信号转导、代谢、转运和转录调控。每个对照组的大多数DEG都是唯一的。我们观察到在Y 58 S对照组中有更多差异表达的MYB（成髓细胞瘤）和锌指家族转录因子和信号转导组分，如钙调蛋白/钙依赖性蛋白激酶。核糖体相关的DEGs可能在冷胁迫信号转导中起重要作用。这些结果对进一步研究水稻对冷胁迫反应的分子机制具有重要意义。
Rice is highly sensitive to cold stress during reproductive developmental stages, and little is known about the mechanisms of cold responses in rice anther. Using the HiSeq 2000 sequencing platform, the anther transcriptome of photo thermo sensitive genic male sterile lines (PTGMS) rice Y58S and P64S (Pei'ai64S) were analyzed at the fertility sensitive stage under cold stress. Approximately 243 million clean reads were obtained from four libraries and aligned against the oryza indica genome and 1497 and 5652 differentially expressed genes (DEGs) were identified in P64S and Y58S, respectively. Both gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were conducted for these DEGs. Functional classification of DEGs was also carried out. The DEGs common to both genotypes were mainly involved in signal transduction, metabolism, transport, and transcriptional regulation. Most of the DEGs were unique for each comparison group. We observed that there were more differentially expressed MYB (Myeloblastosis) and zinc finger family transcription factors and signal transduction components such as calmodulin/calcium dependent protein kinases in the Y58S comparison group. It was also found that ribosome-related DEGs may play key roles in cold stress signal transduction. These results presented here would be particularly useful for further studies on investigating the molecular mechanisms of rice responses to cold stress.