Immunohistological analysis of immune cells in blistering skin lesions
Immunohistological analysis of immune cells in blistering skin lesions
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DOI：
10.1136/jcp.2006.037010
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发表时间：
2007-01-01
影响因子：
3.4
通讯作者：
Omar, Abd-Elhady M. M.
中科院分区：
文献类型：
--
作者：
Hussein, Mahmoud R.;Ali, Fayed Mahammad Nagy;Omar, Abd-Elhady M. M.
背景：大疱性皮肤病变的特征是存在表皮内或表皮下大疱。尽管炎症细胞浸润在这些病变中是一个恒定的特征，但它们的免疫表型特征仍然不完整。目的：探讨大疱性皮肤病的发展是否与炎性细胞浸润的改变有关。材料和方法：采用免疫过氧化物酶染色法和针对组织细胞（CD68）、B细胞（CD20+）、T细胞（CD3+）、具有细胞毒性的T细胞（T细胞胞内相关抗原，TIA1+）和活性（颗粒酶B， GRB+）抗原的抗体检测34例表皮内和皮下大泡病变。邻近的正常皮肤（病变）和另外5例正常皮肤也被检查（对照组）。结果：从正常皮肤到病变皮肤（表皮内和表皮下大泡病变）的转变与显著增加相关(p
Background: Bullous skin lesions are characterised by the presence of intraepidermal or subepidermal bullae. Although inflammatory cell infiltrate is a constant feature in these lesions, their immunophenotypic characterisation is still incomplete.Aim: To determine whether the development of bullous skin diseases is associated with changes in the inflammatory cell infiltrate.Materials and methods: 34 cases representing lesions with both intraepidermal and subepidermal bullae were examined using immunoperoxidase staining methods and antibodies targeting antigens for histiocytes (CD68), B cells (CD20+), T cells (CD3+), T cells with cytotoxic potential ( T cell intracellular associated antigen,TIA1+) and activity ( granzyme B, GRB+). The adjacent normal skin ( lesions) and an additional five cases of normal skin were also examined ( controls).Results: The transition from normal skin to lesional skin ( lesions with intraepidermal and subepidermal bullae) was associated with a significant increase (p