Multi-channel 3-D cell culture device integrated on a silicon chip for anticancer drug sensitivity test
Multi-channel 3-D cell culture device integrated on a silicon chip for anticancer drug sensitivity test
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DOI：
10.1016/j.biomaterials.2004.05.028
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发表时间：
2005-05-01
期刊：
影响因子：
14
通讯作者：
Matsue, T
中科院分区：
文献类型：
--
作者：
Torisawa, Y;Shiku, H;Matsue, T
构建了一种新型的三维细胞培养系统，该系统在硅芯片中具有细胞板阵列（4 × 5）。以及多通道药物容器。将人乳腺癌（MCF-7）细胞包埋在胶原凝胶基质中，并包埋在一个圆形的硅孔中。每个细胞板可以通过由微流体部分和储器组成的通道隔离。将细胞板暴露于200 mm KCN 2天，以证明每个细胞板可以在各种刺激条件下独立评价。基于细胞呼吸活性，使用扫描电化学显微镜（SECM）在硅基细胞阵列上连续监测增殖行为5天。在装置中捕获的细胞（3-D培养）正常增殖，并且增殖率低于在单层细胞培养（2-D培养）中生长的细胞。三种抗癌药物同时测量的细胞芯片上的效果是在良好的协议，通过传统的比色测定。我们的研究结果表明，基于硅的三维培养装置是适当的化疗敏感性测定，涉及多化学刺激。(C)2004爱思唯尔有限公司保留所有权利。
A novel three-dimensional cell culture system was constructed with an array of cell panels (4 x 5) in a silicon chip. together with multi-channel drug containers. Human breast cancer (MCF-7) cells were embedded in a collagen-gel matrix and entrapped in a pyramidal-shaped silicon hole. Each cell panel can be isolated by a channel composed of a microfluid part and a reservoir. A cell panel was exposed to 200 mm KCN for 2 days to demonstrate that each cell panel could be independently evaluated under various stimulation conditions. Based on the cellular respiration activity, the proliferation behavior was continuously monitored on the silicon-based cell array for 5 days using scanning electrochemical microscopy (SECM). The cells entrapped in the device (3-D culture) proliferated normally, and the proliferation rate was lower than that of cells grown in a monolayer cell culture (2-D culture). The effects of three anticancer drugs measured simultaneously on the cell chip were in good agreement with those obtained by a conventional colorimetric assay. Our results suggest that the silicon-based device for 3D culture is appropriate for a chemosensitivity assay involving multi-chemical stimulation. (C) 2004 Elsevier Ltd. All rights reserved.