Detection of Interferon gamma using graphene and aptamer based FET-like electrochemical biosensor
Detection of Interferon gamma using graphene and aptamer based FET-like electrochemical biosensor
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DOI：
10.1016/j.bios.2015.04.047
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发表时间：
2015-09-15
影响因子：
12.6
通讯作者：
Stroscio, Michael A.
中科院分区：
文献类型：
--
作者：
Farid, Sidra;Meshik, Xenia;Stroscio, Michael A.
科学界的主要目标之一是用于医学诊断和生物医学应用的蛋白质的特异性检测。干扰素-γ（IFN-γ）与结核病易感性有关，结核病是全球主要的健康问题之一。因此，我们开发了一种基于DNA适体的电化学生物传感器，用于高选择性和高灵敏度地检测IFN-γ。基于石墨烯单层的 FET 样结构被整合在 PDMS 基底上，其中 IFN-γ 适体附着在石墨烯上。目标分子的添加会引起电解质中电荷分布的变化，从而导致电子转移效率的增加，这是通过监测设备电流的变化来主动感知的。电流的变化似乎对从纳摩尔 (nM) 到微摩尔 (μM) 范围内的 IFN-γ 浓度高度敏感。我们的 IFN-γ 电化学生物传感器的检测限为 83 pM。通过原子力显微镜使用独特的结构方法验证了适体在石墨烯表面上的固定。这种简单而灵敏的电化学生物传感器在未来的传染病监测、免疫学和癌症研究中具有潜在的应用。 (C) 2015 Elsevier B.V. 保留所有权利。
One of the primary goals in the scientific community is the specific detection of proteins for the medical diagnostics and biomedical applications. Interferon-gamma (IFN-gamma) is associated with the tuberculosis susceptibility, which is one of the major health problems globally. We have therefore developed a DNA aptamer-based electrochemical biosensor that is used for the detection of IFN-gamma with high selectivity and sensitivity. A graphene monolayer-based FET-like structure is incorporated on a PDMS substrate with the IFN-gamma aptamer attached to graphene. Addition of target molecule induces a change in the charge distribution in the electrolyte, resulting in increase in electron transfer efficiency that was actively sensed by monitoring the change in current from the device. Change in current appears to be highly sensitive to the IFN-gamma concentrations ranging from nanomolar (nM) to micromolar (mu M) range. The detection limit of our IFN-gamma electrochemical biosensor is found to be 83 pM. Immobilization of aptamer on graphene surface is verified using unique structural approach by Atomic Force Microscopy. Such simple and sensitive electrochemical biosensor has potential applications in infectious disease monitoring, immunology and cancer research in the future. (C) 2015 Elsevier B.V. All rights reserved.