Actinomycin D induced DNase I cleavage enhancement caused by sequence specific propagation of an altered DNA structure.
Actinomycin D induced DNase I cleavage enhancement caused by sequence specific propagation of an altered DNA structure.
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Actinomycin D 诱导 DNase I 裂解增强，这是由改变的 DNA 结构的序列特异性传播引起的。
DOI：
10.1093/nar/16.23.11125
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发表时间：
1988
影响因子：
14.9
通讯作者：
Lane,MJ
中科院分区：
文献类型：
--
作者：
Huang,YQ;Rehfuss,RP;LaPlante,SR;Boudreau,E;Borer,PN;Lane,MJ
在放线菌素d存在和不存在的情况下，两个含有一个中心5 ‘ -GC-3 ’碱基步骤的DNA十六进制体已经通过足迹方法进行了检查。结合亚质子核磁共振测量表明，抗肿瘤药物引起距离d序列放线菌素嵌入位点一定距离的DNA构象发生变化[ATATATAGCTATATAT]，而在d序列[AAAAAAAGCTTTTTTT]中不会发生这种变化。实验表明，与放线菌素d结合相关的DNA酶I速率增强是由配体改变平衡DNA结构引起的。
Two DNA hexadecamers containing one central 5′-GC-3′ base step have been examined by footprinting methodology in the presence and absence of actinomycin D. The results of these studies, coupled with imino proton NMR measurements indicate that the antitumor drug causes a change in DNA conformation at a distance from the actinomycin intercalation site in a molecule of sequence d[ATATATAGCTATATAT] that does not occur in d[AAAAAAAGCTTTTTTT] The experiments demonstrate that DNase I rate enhancements associated with actinomycin D binding are caused by ligand alteration of equilibrium DNA structure.