Transcriptional regulation of FKLF-2 (KLF13) gene in erythroid cells
Transcriptional regulation of FKLF-2 (KLF13) gene in erythroid cells
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DOI：
10.1016/j.bbaexp.2004.12.007
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发表时间：
2005-02-14
期刊：
影响因子：
--
通讯作者：
Naoe, T
中科院分区：
文献类型：
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作者：
Mitsuma, A;Asano, H;Naoe, T
FKLF-2(KLF13)是从胎儿珠蛋白表达的组织中克隆的，已被证明在红系细胞中大量表达。在这项研究中，我们检测了KLF13基因的转录调控。从小鼠红细胞白血病(Mel)细胞基因组DNA中克隆了一个5.5kb的5‘侧翼区，结果表明该启动子在K562细胞中的活性高于在COS-7细胞中的活性。该启动子被共表达的GATA-1通过含有两个CCAAT基序的序列反式激活，表明GATA-1参与了KLF13 m.RNA在红系组织中的丰富表达。在其自身的启动子上观察到KLF13基因在COS-7中的激活作用和在K562细胞中的抑制作用，提示KLF13基因在红系环境中的转录调控存在反馈机制。这些发现为了解KLF13基因在红系细胞中可诱导表达的机制提供了新的思路。(C)2004爱思唯尔B.V.保留所有权利。
FKLF-2 (KLF13) was cloned from fetal globin-expressing tissues and has been shown to be abundantly expressed in erythroid cells. In this study we examined the transcriptional regulation of the KLF13 gene. A 5.5 kb 5' flanking region cloned from mouse erythroleukerriia (MEL) cell genomic DNA showed that major cis regulatory activities exist in the 550 bp sequence to the unique transcription start site, and that the promoter is more active in K562 cells than in COS-7 cells. The promoter was trans-activated by co-expressed GATA-1 through the sequence containing two CCAAT motifs, suggesting that GATA-1 is involved in the abundant expression of KLF13 m.RNA in the erythroid tissue. Dual action, i.e. activating effect in COS-7 and repressive effect in K562 cell, was observed on its own promoter, suggesting a feedback mechanism for the transcriptional control of the KLF13 gene in the erythroid environment. These findings provide an insight on the mechanism of inducible mRNA expression of the KLF13 gene in erythroid cells. (C) 2004 Elsevier B.V. All rights reserved.