Membrane-structure studies using X-ray standing waves.
Membrane-structure studies using X-ray standing waves.
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使用 X 射线驻波进行膜结构研究。
DOI：
10.1146/annurev.bb.24.060195.002031
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Wang,J
中科院分区：
文献类型：
--
作者：
Caffrey,M;Wang,J
本文介绍了X射线驻波法研究膜结构的原理和特点。到目前为止，X射线驻波已被用于Langmuir-Blodgett和自组装单分子脂质膜的结构研究。最近，该方法已被用于研究支持膜托管外周膜蛋白，细胞色素c。发生在膜和表面的温度和组成的变化驱动的结构重排也被监测。最后，离子分布在扩散双电层旁边的带电膜已确定使用这种方法。审查涉及进行这些和相关测量的方式。什么是不包括在审查是一个关键的评估的局限性的X射线驻波法。这种局限性（在膜蛋白拓扑学研究中使用的银镜上，这种局限性是明显的，并在短距离内引入了显著的测量模糊性）最近才引起我们的注意，并将单独报道（S Kirchner，Z Yin，J Wang和M Caffrey，准备中）。
The principle and unique features of X-ray standing waves as a means for investigating membrane structure are described in this review. Thus far, X-ray standing waves have been used in structural studies of Langmuir-Blodgett and self-assembled monomolecular lipid films. Most recently, the method has been used in studies of supported membranes hosting the peripheral membrane protein, cytochrome c. Structural rearrangements occurring in membranes and at surfaces driven by temperature and composition changes have been monitored as well. Finally, ion distribution in the diffuse-double layer next to a charged membrane has been determined using this approach. The review addresses the manner in which these and related measurements were made. What is not covered in the review is a critical appraisal of the limitations of the X-ray standing wave method. Such limitations (which are pronounced and introduce significant mensuration ambiguities at short distances on silver mirrors as used in the membrane protein topology study) have just recently come to our attention and will be reported on separately (S Kirchner, Z Yin, J Wang & M Caffrey, in preparation).