Synthesis of amine-functionalized diene-based polymers as novel gene delivery vectors
Synthesis of amine-functionalized diene-based polymers as novel gene delivery vectors
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DOI：
10.1021/ma061249p
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发表时间：
2006-11
期刊：
影响因子：
5.5
通讯作者：
Yue Yang;Jin Lee;M. Cho;V. Sheares
中科院分区：
文献类型：
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作者：
Yue Yang;Jin Lee;M. Cho;V. Sheares
通过阴离子和自由基聚合技术合成了一系列胺官能化的基于二烯的聚合物，以评估它们作为基因递送载体的潜力。检查细胞毒性、结合亲和力和转染效率特性。结构-性能关系的系统研究表明，由于质子化后酸性降低，聚合物的侧基从甲基变为丙基，细胞毒性降低。聚合物和DNA之间的结合亲和力也随着侧基的大小的增加而降低。在所研究的聚合物中，聚[2-（N，N-二乙基氨基甲基）-1，3-丁二烯]在N/P比为2时具有比聚乙烯亚胺更低的细胞毒性和更高的转染效率，是一种有前途的新型转染载体。
A series of amine-functionalized diene-based polymers were synthesized via anionic and free radical polymerization techniques for evaluation of their potential as gene delivery vectors. Cytotoxicity, binding affinity, and transfection efficiency properties were examined. A systematic study of the structure−property relationships revealed that cytotoxicity decreased as the side group of the polymers changed from methyl to propyl group due to the decrease of the acidity after protonation. The binding affinity between the polymers and DNA also decreased as the size of the side group increased. Of the polymers studied, poly[2-(N,N-diethylaminomethyl)-1,3-butadiene], with lower cytotoxicity than poly(ethylene imine) and higher transfection efficiency at an N/P ratio of 2, is a promising, novel transfection vector.