Novel magnetic calcium phosphate-stem cell construct with magnetic field enhances osteogenic differentiation and bone tissue engineering.
Novel magnetic calcium phosphate-stem cell construct with magnetic field enhances osteogenic differentiation and bone tissue engineering.
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DOI：
10.1016/j.msec.2018.12.120
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发表时间：
2019-05
期刊：
影响因子：
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通讯作者：
Yang Xia;Huimin Chen;Yantao Zhao;Feimin Zhang;Xiaodong Li;Lin Wang;M. Weir;Junqing Ma;
中科院分区：
文献类型：
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作者：
Yang Xia;Huimin Chen;Yantao Zhao;Feimin Zhang;Xiaodong Li;Lin Wang;M. Weir;Junqing Ma;
超顺磁性氧化铁纳米粒子（IONP）是一种很有前途的生物活性添加剂，可用于构建骨组织工程磁性支架。到目前为止，还没有关于IONP掺入的磷酸钙骨水泥（IONP-CPC）支架在使用外部静磁场（SMF）的干细胞上的骨诱导性的报道。本研究的目的是：（1）构建一种新型的磁性IONP-CPC骨组织工程支架;（2）研究IONP-CPC复合物和SMF对人牙髓干细胞（hDPSCs）增殖、成骨分化和骨矿物质合成的影响。与CPC对照组和ILONP-CPC相比，SMF下的新型磁性IONP-CPC增强了hDPSC的细胞性能，产生更大的碱性磷酸酶活性（约3倍），成骨标记基因的表达增加，以及更多的细胞合成的骨矿物质（约2.5倍）。此外，在大鼠下颌骨缺损中，IONP-CPC比CPC对照诱导更活跃的成骨。这些结果与SMF下培养基中磁性IONP增强的细胞性能一致。此外，通过透射电子显微镜（TEM）在细胞内检测到纳米聚集体。因此，增强的细胞性能归因于由磁场产生的物理力以及从IONP-CPC构建体释放的磁性纳米颗粒的细胞内化。
Superparamagnetic iron oxide nanoparticles (IONPs) are promising bioactive additives to fabricate magnetic scaffolds for bone tissue engineering. To date, there has been no report on osteoinductivity of IONP-incorporated calcium phosphate cement (IONP-CPC) scaffold on stem cells using an exterior static magnetic field (SMF). The objectives of this study were to: (1) develop a novel magnetic IONP-CPC construct for bone tissue engineering, and (2) investigate the effects of IONP-incorporation and SMF application on the proliferation, osteogenic differentiation and bone mineral synthesis of human dental pulp stem cells (hDPSCs) seeded on IONP-CPC scaffold for the first time. The novel magnetic IONP-CPC under SMF enhanced the cellular performance of hDPSCs, yielding greater alkaline phosphatase activities (about 3-fold), increased expressions of osteogenic marker genes, and more cell-synthesized bone minerals (about 2.5-fold), compared to CPC control and nonmagnetic IONP-CPC. In addition, IONP-CPC induced more active osteogenesis than CPC control in rat mandible defects. These results were consistent with the enhanced cellular performance by magnetic IONP in media under SMF. Moreover, nano-aggregates were detected inside the cells by transmission electron microscopy (TEM). Therefore, the enhanced cell performance was attributed to the physical forces generated by the magnetic field together with cell internalization of the released magnetic nanoparticles from IONP-CPC constructs.