Short-lived plasma blasts are the main B cell effector subset during the course of multiple sclerosis
Short-lived plasma blasts are the main B cell effector subset during the course of multiple sclerosis
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DOI：
10.1093/brain/awh486
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发表时间：
2005-07-01
期刊：
影响因子：
14.5
通讯作者：
Hemmer, B
中科院分区：
文献类型：
--
作者：
Cepok, S;Rosche, B;Hemmer, B
多发性硬化症是一种病因不明的中枢神经系统慢性炎症和脱髓鞘疾病。尽管鞘内免疫球蛋白 G (IgG) 合成是该疾病的一个关键特征，但对于多发性硬化症患者中枢神经系统中 B 细胞的反应仍知之甚少。我们分析了多发性硬化症、传染病 (IND) 和非炎症性神经系统疾病 (NIND) 患者中不同 B 细胞亚群的表型和动力学。在多发性硬化症和 IND 患者的脑脊液中检测到 B 细胞，但在 NIND 患者中基本上不存在。在脑脊液中，大多数 B 细胞具有记忆 B 细胞和短命浆母细胞 (PB) 的表型；隔室中不存在浆细胞。多发性硬化症患者和急性中枢神经系统感染患者的PB比例最高。虽然 IND 患者感染消退后 PB 迅速从 CSF 中消失，但这些细胞在多发性硬化症患者的整个病程中大量存在。 MRI 揭示，多发性硬化症患者的 CSF PB 数量与鞘内 IgG 合成和炎症性实质疾病活动密切相关。这项研究将短命浆母细胞确定为参与多发性硬化症患者持续活动性炎症的主要效应 B 细胞群。
Multiple sclerosis is a chronic inflammatory and demyelinating disorder of the CNS with an unknown aetiology. Although intrathecal immunoglobulin G (IgG) synthesis is a key feature of the disease, little is still known about the B cell response in the CNS of multiple sclerosis patients. We analysed the phenotype and kinetics of different B cell subsets in patients with multiple sclerosis, infectious disease (IND) and non-inflammatory neurological disease (NIND). B cells were detected in the CSF of multiple sclerosis and IND patients, but were largely absent in NIND patients. In the CSF, the majority of B cells had a phenotype of memory B cells and short-lived plasma blasts (PB); plasma cells were absent from the compartment. The proportion of PB was highest in multiple sclerosis patients and patients with acute CNS infection. While PB disappeared rapidly from the CSF after resolution of infection in IND patients, these cells were present at high numbers throughout the disease course in multiple sclerosis patients. CSF PB numbers in multiple sclerosis patients strongly correlated with intrathecal IgG synthesis and inflammatory parenchymal disease activity as disclosed by MRI. This study identifies short-lived plasma blasts as the main effector B cell population involved in ongoing active inflammation in multiple sclerosis patients.