Assessing redox state and reactive oxygen species in circadian rhythmicity.
Assessing redox state and reactive oxygen species in circadian rhythmicity.
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DOI：
10.1007/978-1-4939-0700-7_17
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发表时间：
2014
影响因子：
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通讯作者：
Katharina König;Helena Galliardt;M. Moore;P. Treffon;T. Seidel;K. Dietz
中科院分区：
文献类型：
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作者：
Katharina König;Helena Galliardt;M. Moore;P. Treffon;T. Seidel;K. Dietz
氧化还原稳态是细胞功能和细胞信号传导的重要参数。氧化还原状态的空间和时间变化控制新陈代谢、发育过程以及对环境应激和应激适应的急性反应。氧化还原稳态也与生物钟有关。本章介绍了评估重要氧化还原参数的方法，例如低分子量氧化还原代谢物谷胱甘肽和抗坏血酸、它们的量和氧化还原状态，以及作为活性氧的 H2O2。体内氧化还原细胞成像是通过使用氧化还原敏感的绿色荧光蛋白（roGFP）来描述的。最后，在蛋白质翻译后氧化还原修饰水平上，显示了评估 2-半胱氨酸过氧化还原蛋白过度氧化和过氧化还原蛋白 IIE 谷胱甘肽化的方法。 2-半胱氨酸过氧化还原蛋白的氧化还原状态已被确定为昼夜节律的转录独立标记。
Redox homeostasis is an important parameter of cell function and cell signaling. Spatial and temporal alterations of redox state control metabolism, developmental processes, as well as acute responses to environmental stresses and stress acclimation. Redox homeostasis is also linked to the circadian clock. This chapter introduces methods to assess important redox parameters such as the low molecular weight redox metabolites glutathione and ascorbate, their amount and redox state, and H2O2as reactive oxygen species. In vivo redox cell imaging is described by use of the reduction–oxidation sensitive green fluorescent protein (roGFP). Finally, on the level of posttranslational redox modifications of proteins, methods are shown to assess hyperoxidation of 2-cysteine peroxiredoxin and glutathionylation of peroxiredoxin IIE. The redox state of 2-cysteine peroxiredoxin has been identified as a transcription-independent marker of circadian rhythmicity.