Profiling of Serum Bile Acids in a Healthy Chinese Population Using UPLC-MS/MS
Profiling of Serum Bile Acids in a Healthy Chinese Population Using UPLC-MS/MS
复制标题
DOI：
10.1021/pr500920q
复制
发表时间：
2015-02-01
影响因子：
4.4
通讯作者：
Jia, Wei
中科院分区：
文献类型：
--
作者：
Xie, Guoxiang;Wang, Yixing;Jia, Wei
胆汁酸（BAs）是胆固醇代谢、肠道营养吸收、胆汁分泌脂类、有毒代谢物和外源物的重要生理制剂。近二十年来的广泛研究揭示了BAs作为信号分子和代谢调节剂的新功能，通过激活核受体和肠-肝代谢轴中涉及宿主-肠道微生物共代谢的g蛋白偶联受体信号，调节肝脏脂质、葡萄糖和能量稳态。因此，在不同年龄和体重指数（BMI）的健康男性和女性受试者中进行血清BA谱的研究，将为研究受宿主基因组和共生微生物组两套基因组调控的人类受试者的BA生理和代谢稳态提供重要的基线信息。以往关于动物和人类BA谱与年龄或性别相关变化的报告显示结果不一致，并且从各种研究中获得的信息高度不完整。在这里，我们分析了大量健康参与者（n = 502）的血清BA，并使用超高效液相色谱三重四极杆质谱法检测了年龄、性别和BMI对血清BA浓度和组成的影响。我们发现研究对象的BA谱依赖于性别、年龄和BMI。男性总BAs显著高于女性（p < 0.05），肥胖女性总BAs显著高于瘦弱女性（p < 0.05）。在50-70岁年龄段，男女受试者之间BA谱的差异减小，而在50-70岁年龄段，瘦与肥胖受试者之间BA谱的差异增大。该研究提供了对健康受试者BA谱的全面了解，并强调在调查胃肠道疾病引起的BA病理生理变化时，需要考虑年龄、性别和BMI差异。
Bile acids (BAs) are a group of important physiological agents for cholesterol metabolism, intestinal nutrient absorption, and biliary secretion of lipids, toxic metabolites, and xenobiotics. Extensive research in the last two decades has unveiled new functions of BAs as signaling molecules and metabolic regulators that modulate hepatic lipid, glucose, and energy homeostasis through the activation of nuclear receptors and G-protein-coupled receptor signaling in gut-liver metabolic axis involving host-gut microbial co-metabolism. Therefore, investigation of serum BA profiles, in healthy human male and female subjects with a wide range of age and body mass index (BMI), will provide important baseline information on the BA physiology as well as metabolic homeostasis among human subjects that are regulated by two sets of genome, host genome, and symbiotic microbiome. Previous reports on age- or gender-related changes on BA profiles in animals and human showed inconsistent results, and the information acquired from various studies was highly fragmentary. Here we profiled the serum BAs in a large population of healthy participants (n = 502) and examined the impact of age, gender, and BMI on serum BA concentrations and compositions using a targeted metabonomics approach with ultraperformance liquid chromatography triple-quadrupole mass spectrometry. We found that the BA profiles were dependent on gender, age, and BMI among study subjects. The total BAs were significantly higher in males than in females (p < 0.05) and higher in obese females than in lean females (p < 0.05). The difference in BA profiles between male and female subjects was decreased at age of 50-70 years, while the difference in BA profiles between lean and obese increased for subjects aged 50-70 years. The study provides a comprehensive understanding of the BA profiles in healthy subjects and highlights the need to take into account age, gender, and BMI differences when investigating pathophysiological changes of BAs resulting from gastrointestinal diseases.