Emergence of ST147 Klebsiella pneumoniae Producing OXA-204 Carbapenemase in a University Hospital, Tunisia.
Emergence of ST147 Klebsiella pneumoniae Producing OXA-204 Carbapenemase in a University Hospital, Tunisia.
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突尼斯大学医院出现生产 OXA-204 碳青霉烯酶的 ST147 肺炎克雷伯菌。
DOI：
10.1089/mdr.2014.0278
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
MadecJean
中科院分区：
文献类型：
--
作者：
GramiRaoudha;MansourWejdene;Ben Haj KhalifaAnis;DahmenSafia;ChâtrePierre;HaenniMarisa;AouniMahjoub;MadecJean
研究了突尼斯塔哈尔斯法大学医院首次发现的耐碳青霉烯肺炎克雷伯菌分离株（KP1和KP2）的分子特征。进行药敏试验、多位点序列分型、S1核酸酶脉冲场凝胶电泳、Southern blot和基于聚合酶链反应（PCR）的复制子分型。采用PCR和测序方法检测β-内酰胺酶和碳青霉烯酶的广谱基因。两株菌株均具有多重耐药。KP1序列型（ST） ST101，在不可分型质粒上显示blaCTX-M-15和blem -1，在IncL/M质粒上显示blaOXA-48。KP2与KP1 （ST147）在遗传上无关，并且携带一个携带blaCMY-4和blaOXA-48衍生基因blaOXA-204的IncA/C质粒。本研究报告了世界范围内来自同一国家的产生oxa -204的肺炎克雷伯菌分离株的第二例病例，但具有不同的遗传背景。
Molecular features of the first carbapenem-resistant Klebsiella pneumoniae isolates (KP1 and KP2) from the University Hospital Tahar Sfar, Tunisia, were investigated. Antimicrobial susceptibility testing, multilocus sequence typing, S1 nuclease pulsed-field gel electrophoresis, Southern blot, and polymerase chain reaction (PCR)-based replicon typing were performed. Extended-spectrum β-lactamases and carbapenemase genes were detected by PCR and sequencing. Both isolates were multidrug resistant. KP1 was of sequence type (ST) ST101 and exhibited blaCTX-M-15 and blaTEM-1 on an untypeable plasmid and blaOXA-48 on an IncL/M plasmid. KP2 was genetically unrelated to KP1 (ST147) and harbored an IncA/C plasmid carrying blaCMY-4 and the blaOXA-48 derivative gene: blaOXA-204. This study reports the second case worldwide of an OXA-204-producing K. pneumoniae isolate from the same country, however, in a different genetic background.