Bioceramic-collagen scaffolds loaded with human adipose-tissue derived stem cells for bone tissue engineering
Bioceramic-collagen scaffolds loaded with human adipose-tissue derived stem cells for bone tissue engineering
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DOI：
10.1007/s11033-013-2913-8
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发表时间：
2014-02-01
影响因子：
2.8
通讯作者：
Norouzian, Mohsen
中科院分区：
文献类型：
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作者：
Daei-farshbaf, Neda;Ardeshirylajimi, Abdolreza;Norouzian, Mohsen
生物陶瓷与干细胞的结合引起了研究界对骨组织工程应用的兴趣。在本研究中，Bio-Oss（A（R））和1型胶原凝胶的组合作为支架，在分离和表征后负载人脂肪组织来源的间充质干细胞（AT-MSC），并使用数字乳腺X线摄影、多层螺旋计算机断层扫描成像和组织学分析研究它们在大鼠临界尺寸缺损中的骨再生能力。植入后8周，缺损部位未观察到死亡或炎症迹象。根据成像分析结果，与未治疗组相比，接受Bio-Oss(A(R))-Gel的大鼠观察到更高水平的骨再生。此外，在所有组中，接种 MSC 的 Bio-Oss-Gel 诱导的骨重建最高。组织学染色证实了这些发现，并且与 Bio-Oss-Gel 相比，在 MSC 接种的 Bio-Oss-Gel 中观察到了令人印象深刻的骨整合。总的来说，AT-MSCs、Bio-Oss和Gel的组合可以协同增强骨再生和重建，也可以作为骨再生医学和组织工程应用的合适结构。
The combination of bioceramics and stem cells has attracted the interest of research community for bone tissue engineering applications. In the present study, a combination of Bio-Oss(A (R)) and type 1 collagen gel as scaffold were loaded with human adipose-tissue derived mesenchymal stem cells (AT-MSCs) after isolation and characterization, and the capacity of them for bone regeneration was investigated in rat critical size defects using digital mammography, multi-slice spiral computed tomography imaging and histological analysis. 8 weeks after implantation, no mortality or sign of inflammation was observed in the site of defect. According to the results of imaging analysis, a higher level of bone regeneration was observed in the rats receiving Bio-Oss(A (R))-Gel compared to untreated group. In addition, MSC-seeded Bio-Oss-Gel induced the highest bone reconstruction among all groups. Histological staining confirmed these findings and impressive osseointegration was observed in MSC-seeded Bio-Oss-Gel compared with Bio-Oss-Gel. On the whole, it was demonstrated that combination of AT-MSCs, Bio-Oss and Gel synergistically enhanced bone regeneration and reconstruction and also could serve as an appropriate structure to bone regenerative medicine and tissue engineering application.