Carbon dots as inhibitors of virus by activation of type I interferon response
Carbon dots as inhibitors of virus by activation of type I interferon response
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碳点通过激活 I 型干扰素反应作为病毒抑制剂
DOI：
10.1016/j.carbon.2016.09.032
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发表时间：
2016-12-01
期刊：
影响因子：
10.9
通讯作者：
Han, Heyou
中科院分区：
文献类型：
--
作者：
Du, Ting;Liang, Jiangong;Han, Heyou
碳点（CD）由于其低毒性、生物和生态友好性以及量子点（QD）的理想性能特征，在生物科学和生物技术领域显示出令人兴奋的潜力。然而，CD 对病毒的影响仍然很大程度上未知。在本研究中，我们分别以伪狂犬病病毒（PRV）和猪繁殖与呼吸综合征病毒（PRRSV）作为DNA病毒和RNA病毒模型，研究CD对病毒复制的影响。病毒滴度和病毒蛋白表达的分析表明，用CD处理的细胞可以显着抑制PRV和PRRSV的增殖。在机制上，CD 治疗可显着诱导干扰素-α (IFN-α) 的产生和 IFN 刺激基因 (ISG) 的表达，从而抑制病毒复制。总而言之，这些结果揭示了 CD 先前未定义的作用，并提供了开发抗病毒药物的新策略。 (C) 2016 Elsevier Ltd. 保留所有权利。
Carbon dots (CDs) has shown exciting potential in the field of bioscience and biotechnology due to their low toxicity, biological and ecological friendliness and desirable performance characteristics of quantum dots (QDs). However, the effects of CDs on viruses are still largely unknown. In this study, we investigate the effect of CDs on the replication of virus by using pseudorabies virus (PRV) and porcine reproductive and respiratory syndrome virus (PRRSV) as the models of DNA virus and RNA virus, respectively. Analyses of virus titers and the expression of viral proteins demonstrate that cells treated with CDs can significantly inhibit the multiplication of PRV and PRRSV. In mechanism, CDs treatment dramatically induces interferon-alpha (IFN-alpha) production and the expression of IFN-stimulating genes (ISGs) which in turn inhibits virus replication. Taken together, these results reveal a previously undefined role of CDs and provide a new strategy to develop antiviral agents. (C) 2016 Elsevier Ltd. All rights reserved.